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Merck

68268

Imidazole buffer Solution

BioUltra, 1 M in H2O

동의어(들):

Imidazole solution, Glyoxaline solution

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제품정보 (DICE 배송 시 비용 별도)

실험식(Hill 표기법):
C3H4N2
CAS 번호:
Molecular Weight:
68.08
UNSPSC Code:
12161700
NACRES:
NA.25
PubChem Substance ID:
MDL number:
Beilstein/REAXYS Number:
103853
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제품 이름

Imidazole buffer Solution, BioUltra, 1 M in H2O

InChI key

RAXXELZNTBOGNW-UHFFFAOYSA-N

InChI

1S/C3H4N2/c1-2-5-3-4-1/h1-3H,(H,4,5)

SMILES string

c1c[nH]cn1

product line

BioUltra

form

liquid

concentration

1 M in H2O

impurities

DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected

pH

9.5±0.5

density

1.01 g/mL at 20 °C

Quality Level

Application

Imidazole buffer solution has been used as a component:
  • of calmodulin-binding buffer 1 (CBB1) for the tandem affinity purification (TAP) of TAP-tagged proteins together with co-purifying proteins
  • of lysis buffer, and SDS loading buffer for His-ubiquitin pull-down assays
  • of imidazole wash and elution buffer for protein purification

General description

Imidazole is used as a buffer in the pH range of 6.2-7.8 at 25°C. It is recommended as a component of a buffer for the assay of horseradish peroxidase and as a chelator for the binding of various divalent cations. Imidazole aids the elution of histidine-containing proteins from divalent cation resins (Sigma P6611, HisSelect.-HC Nickel affinity gel) and can also be used in reverse staining of SDS-PAGE gels for the detection of proteins.

pictograms

Health hazardCorrosion

signalword

Danger

Hazard Classifications

Eye Dam. 1 - Repr. 1B - Skin Corr. 1C

저장 등급

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter


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The Journal of biological chemistry, 290(22), 14077-14090 (2015-04-17)
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Molecular therapy : the journal of the American Society of Gene Therapy, 23(8), 1285-1297 (2015-06-09)
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Proceedings of the National Academy of Sciences of the United States of America, 112(28), E3669-E3678 (2015-07-01)
Acetylation is correlated with chromatin decondensation and transcriptional activation, but its regulation by histone deacetylase (HDAC)-bearing corepressor complexes is poorly understood. Here, we describe the mechanism of assembly of the mammalian Sin3L/Rpd3L complex facilitated by Sds3, a conserved subunit deemed
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The Journal of biological chemistry, 290(37), 22520-22531 (2015-07-30)
CUX1 and CUX2 proteins are characterized by the presence of three highly similar regions called Cut repeats 1, 2, and 3. Although CUX1 is ubiquitously expressed, CUX2 plays an important role in the specification of neuronal cells and continues to

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