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크기 선택
제품정보 (DICE 배송 시 비용 별도)
실험식(Hill 표기법):
C3H4N2
CAS 번호:
Molecular Weight:
68.08
UNSPSC Code:
12161700
NACRES:
NA.25
PubChem Substance ID:
MDL number:
Beilstein/REAXYS Number:
103853
InChI key
RAXXELZNTBOGNW-UHFFFAOYSA-N
InChI
1S/C3H4N2/c1-2-5-3-4-1/h1-3H,(H,4,5)
SMILES string
c1c[nH]cn1
product line
BioUltra
form
liquid
concentration
1 M in H2O
impurities
DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected
pH
9.5±0.5
density
1.01 g/mL at 20 °C
Quality Level
General description
Imidazole is used as a buffer in the pH range of 6.2-7.8 at 25°C. It is recommended as a component of a buffer for the assay of horseradish peroxidase and as a chelator for the binding of various divalent cations. Imidazole aids the elution of histidine-containing proteins from divalent cation resins (Sigma P6611, HisSelect.-HC Nickel affinity gel) and can also be used in reverse staining of SDS-PAGE gels for the detection of proteins.
Application
Imidazole buffer solution has been used as a component:
- of calmodulin-binding buffer 1 (CBB1) for the tandem affinity purification (TAP) of TAP-tagged proteins together with co-purifying proteins
- of lysis buffer, and SDS loading buffer for His-ubiquitin pull-down assays
- of imidazole wash and elution buffer for protein purification
signalword
Danger
hcodes
Hazard Classifications
Eye Dam. 1 - Repr. 1B - Skin Corr. 1C
저장 등급
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter
Peter Canning et al.
Journal of molecular biology, 426(13), 2457-2470 (2014-04-29)
The discoidin domain receptors (DDRs), DDR1 and DDR2, form a unique subfamily of receptor tyrosine kinases that are activated by the binding of triple-helical collagen. Excessive signaling by DDR1 and DDR2 has been linked to the progression of various human
Pam M Van Ry et al.
Molecular therapy : the journal of the American Society of Gene Therapy, 23(8), 1285-1297 (2015-06-09)
Duchenne muscular dystrophy (DMD) is a fatal neuromuscular disease caused by mutations in the dystrophin gene, leading to the loss of a critical component of the sarcolemmal dystrophin glycoprotein complex. Galectin-1 is a small 14 kDa protein normally found in skeletal
Ali Tebbi et al.
The Journal of biological chemistry, 290(22), 14077-14090 (2015-04-17)
Ribonucleotide reductase (RnR) is a key enzyme synthesizing deoxyribonucleotides for DNA replication and repair. In mammals, the R1 catalytic subunit forms an active complex with either one of the two small subunits R2 and p53R2. Expression of R2 is S
William A Gaines et al.
Nature communications, 6, 7834-7834 (2015-07-29)
The conserved budding yeast Rad51 paralogues, including Rad55, Rad57, Csm2 and Psy3 are indispensable for homologous recombination (HR)-mediated chromosome damage repair. Rad55 and Rad57 are associated in a heterodimer, while Csm2 and Psy3 form the Shu complex with Shu1 and
H Nailwal et al.
Cell death & disease, 6, e1768-e1768 (2015-05-23)
The interplay between influenza virus and host factors to support the viral life cycle is well documented. Influenza A virus (IAV) proteins interact with an array of cellular proteins and hijack host pathways which are at the helm of cellular
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