68268
Imidazole buffer Solution
BioUltra, 1 M in H2O
Sinónimos:
Imidazole solution, Glyoxaline solution
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Fórmula empírica (notación de Hill):
C3H4N2
Número CAS:
Peso molecular:
68.08
UNSPSC Code:
12161700
NACRES:
NA.25
PubChem Substance ID:
MDL number:
Beilstein/REAXYS Number:
103853
eCl@ss:
39161001
Servicio técnico
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BioUltra
Quality Segment
form
liquid
concentration
1 M in H2O
impurities
DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected
pH
9.5±0.5
density
1.01 g/mL at 20 °C
SMILES string
c1c[nH]cn1
InChI
1S/C3H4N2/c1-2-5-3-4-1/h1-3H,(H,4,5)
InChI key
RAXXELZNTBOGNW-UHFFFAOYSA-N
General description
Imidazole is used as a buffer in the pH range of 6.2-7.8 at 25°C. It is recommended as a component of a buffer for the assay of horseradish peroxidase and as a chelator for the binding of various divalent cations. Imidazole aids the elution of histidine-containing proteins from divalent cation resins (Sigma P6611, HisSelect.-HC Nickel affinity gel) and can also be used in reverse staining of SDS-PAGE gels for the detection of proteins.
Application
Imidazole buffer solution has been used as a component:
- of calmodulin-binding buffer 1 (CBB1) for the tandem affinity purification (TAP) of TAP-tagged proteins together with co-purifying proteins
- of lysis buffer, and SDS loading buffer for His-ubiquitin pull-down assays
- of imidazole wash and elution buffer for protein purification
signalword
Danger
hcodes
Hazard Classifications
Eye Dam. 1 - Repr. 1B - Skin Corr. 1C
Clase de almacenamiento
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter
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