B8931
Magenta-Gal
reagent for selection of recombinant bacterial clones
동의어(들):
Red-Gal, 5-Bromo-6-chloro-3-indolyl β-D-galactopyranoside
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크기 선택
제품정보 (DICE 배송 시 비용 별도)
실험식(Hill 표기법):
C14H15BrClNO6
CAS 번호:
Molecular Weight:
408.63
NACRES:
NA.85
PubChem Substance ID:
UNSPSC Code:
12352200
MDL number:
제품 이름
Magenta-Gal, reagent for selection of recombinant bacterial clones
InChI
1S/C14H15BrClNO6/c15-6-1-5-8(2-7(6)16)17-3-9(5)22-14-13(21)12(20)11(19)10(4-18)23-14/h1-3,10-14,17-21H,4H2/t10-,11+,12+,13-,14+/m1/s1
SMILES string
OC[C@H]1O[C@H](Oc2c[nH]c3cc(Cl)c(Br)cc23)[C@H](O)[C@@H](O)[C@H]1O
InChI key
CHRVKCMQIZYLNM-HTOAHKCRSA-N
grade
Molecular Biology
sterility
non-sterile
product line
BioReagent
assay
≥98.0% (HPLC)
form
powder
solubility
methanol: soluble
suitability
suitable for substrate for β-galactosidase
storage temp.
−20°C
Quality Level
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Application
Chromogenic substrate for β-galactosidase producing a red or magenta insoluble product.
Magenta-Gal has been used for staining synthetic cytoplasmic or nuclear LacZ mRNA. It has also been used to detect the expression of the EphB1-β-gal fusion protein. ,Magenta-Gal is suitable for use in the selection of recombinant bacterial colonies with the lac+ phenotype.
Biochem/physiol Actions
When Red-Gal is hydrolyzed by β-galactosidase, the resulting product will form a red precipitate. Lac+ colonies grown in the presence of Red-Gal turn an intense red color, allowing for easy differentiation between lac+ and lac- colonies.
General description
Red-Gal is a chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. Red-Gal is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype.
Red-Gal is a chromogenic substrate for b-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. Red-Gal is designed to replace X-Gal in the blue-white selection of recombinant bacterial colonies with the lac+ phenotype.
저장 등급
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Whole-mount in situ hybridization and immunohistochemistry in Xenopus embryos
In Situ Hybridization Methods, 151-167 (2015)
George Chenaux et al.
The European journal of neuroscience, 34(10), 1620-1633 (2011-11-23)
EphB receptor tyrosine kinases direct axonal pathfinding through interactions with ephrin-B proteins following axon-cell contact. As EphB:ephrin-B binding leads to bidirectional signals, the contributions of signaling into the Eph-expressing cell (forward signaling) or the ephrin-expressing cell (reverse signaling) cannot be
Susan C McCutcheon et al.
BMC biology, 8, 89-89 (2010-06-24)
Reporter genes are widely used in biology and only a limited number are available. We present a new reporter gene for the localization of mammalian cells and transgenic tissues based on detection of the bglA (SYNbglA) gene of Caldocellum saccharolyticum
Maria Belen Jaurena et al.
Nature communications, 6, 7476-7476 (2015-06-24)
All cranial placode progenitors arise from a common precursor field anterior to the neural plate, the pre-placodal region (PPR). We showed that transcription factor Zic1, expressed at the anterior neural plate, is necessary and sufficient to promote placode fate. Here
Yeon-Jin Kim et al.
Developmental biology, 397(1), 129-139 (2014-12-03)
Members of the fibroblast growth factor (FGF) family play important roles during various developmental processes including eye development. FRS (FGF receptor substrate) proteins bind to FGFR and serve as adapters for coordinated assembly of multi-protein complexes involved in Ras/MAPK and
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