F1804

ANTI-FLAG^® M2 antibody, Mouse monoclonal

Name: ANTI-FLAG<SUP>®</SUP> M2 antibody, Mouse monoclonal
Brand: SIGMA

1 mg/mL, clone M2, affinity isolated antibody, buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4)

동의어(들):

flag antibody, Anti-ddddk, Anti-dykddddk, Monoclonal ANTI-FLAG^® M2

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크기 선택

제품정보 (DICE 배송 시 비용 별도)

NACRES:

NA.32

UNSPSC Code:

12352203

주요 문서

모든 문서 보기

기술 서비스

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제품 이름

ANTI-FLAG^® M2 antibody, Mouse monoclonal, 1 mg/mL, clone M2, affinity isolated antibody, buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4)

biological source

mouse

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

M2, monoclonal

form

buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4)

purified by

affinity chromatography (affinity tag purification)

species reactivity

all

concentration

1 mg/mL

isotype

IgG1

immunogen sequence

DYKDDDDK

shipped in

wet ice

storage temp.

−20°C

Quality Level

200

Application

For highly sensitive and specific detection of FLAG fusion proteins by immunoblotting, immunoprecipitation (IP) , immunohistochemisty, immunofluorescence and immunocyotchemistry. Optimized for single banded detection of FLAG fusion proteins in mammalian, plant, and bacterial expression systems.

Western Blotting and EIA

Learn more product details in our FLAG^® application portal.

Biochem/physiol Actions

Binding site:N-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-C

General description

The ANTI-FLAG M2 mouse, affinity purified monoclonal antibody binds to fusion proteins containing a FLAG peptide sequence.The antibody recognizes the FLAG peptide sequence at the N-terminus, Met-N-terminus,C-terminus, and internal sites of the fusion protein.

Method of purification - Affinity tag purification

Immunogen

FLAG; peptide sequence DYKDDDDK

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Physical form

Contains 50% glycerol in phosphate buffered saline.

Preparation Note

Immediately before use, dilute in Tris buffered saline (TBS), pH 8.0, with 3% nonfat Milk

Legal Information

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

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저장 등급

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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시험 성적서(COA)

Lot/Batch Number

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COA 검색

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문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Roquin binds microRNA-146a and Argonaute2 to regulate microRNA homeostasis.

Monika Srivastava et al.

Nature communications, 6, 6253-6253 (2015-02-24)

Roquin is an RNA-binding protein that prevents autoimmunity and inflammation via repression of bound target mRNAs such as inducible costimulator (Icos). When Roquin is absent or mutated (Roquin(san)), Icos is overexpressed in T cells. Here we show that Roquin enhances

HSV-2 glycoprotein gD targets the CC domain of tetherin and promotes tetherin degradation via lysosomal pathway

Yalan Liu

Virology, 154-154 (2016)

SENP3-mediated de-conjugation of SUMO2/3 from promyelocytic leukemia is correlated with accelerated cell proliferation under mild oxidative stress.

Yan Han et al.

The Journal of biological chemistry, 285(17), 12906-12915 (2010-02-26)

Small ubiquitin-like modifier (SUMO) 2/3 is known to conjugate to substrates in response to a variety of cellular stresses. However, whether and how SUMO2/3-specific proteases are involved in de-conjugation under cell stress is unclear. Here, we show that low doses

MCPIP1, alias Regnase-1 binds and cleaves mRNA of C/EBP?

Barbara Lipert

PLoS ONE (2017)

ADAR2 regulates RNA stability by modifying access of decay-promoting RNA-binding proteins

Aparna A

Nucleic Acids Research, 45(7), 4189-4201 (2017)

모든 관련된 서류 보기

문서

Fluorescent Multiplex Detection using Antibody Atto Dye Conjugates

Immunoblotting (Western blot transfer) is a common technique in modern proteomics research.

ANTI-FLAG® M2 antibody, Mouse monoclonal

1 mg/mL, clone M2, affinity isolated antibody, buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4)

크기 선택

제품정보 (DICE 배송 시 비용 별도)

주요 문서

속성

제품 이름

biological source

conjugate

antibody form

antibody product type

clone

form

purified by

species reactivity

concentration

isotype

immunogen sequence

shipped in

storage temp.

Quality Level

관련 카테고리

설명

Application

Biochem/physiol Actions

General description

Immunogen

Other Notes

Physical form

Preparation Note

Legal Information

안전성 정보

저장 등급

wgk

flash_point_f

flash_point_c

문서

시험 성적서(COA)

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문서

관련 콘텐츠

기술 서비스

ANTI-FLAG^® M2 antibody, Mouse monoclonal