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Merck

G1544

Anti-GFP, N-terminal antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

동의어(들):

Anti-Green Fluorescent Protein

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제품정보 (DICE 배송 시 비용 별도)

UNSPSC Code:
12352203
NACRES:
NA.56
MDL number:
MFCD05665217
Conjugate:
unconjugated
Clone:
polyclonal
Application:
immunoprecipitation (IP)
western blot
Technique(s):
immunoprecipitation (IP): 1.0-2.5 μg using GFP fusion protein from transfected mammalian cell lysates
western blot: 0.25-0.5 μg/mL using 3-6 ng of purified GFP fusion proteins expressed in mammalian cell extracts
Citations:
300

주요 문서

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제품 이름

Anti-GFP, N-terminal antibody produced in rabbit, ~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

concentration

~1.0 mg/mL

technique(s)

immunoprecipitation (IP): 1.0-2.5 μg using GFP fusion protein from transfected mammalian cell lysates
western blot: 0.25-0.5 μg/mL using 3-6 ng of purified GFP fusion proteins expressed in mammalian cell extracts

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

200

Application

Anti-GFP, N-terminal antibody produced in rabbit has been used:
  • in immunoblotting
  • in the histochemistry and immunohistochemistry
  • in the immunoprecipitation


Biochem/physiol Actions

Green fluorescent protein (GFP) has photochemical and diagnostic applications. It is also used widely in protein dynamics studies. The polyclonal anti-GFP, N-terminal rabbit antibody reacts specifically with GFP fusion proteins.
Specific for GFP fusion proteins.

General description

Green fluorescent protein (GFP) chromophore contains hexapeptide fragment with Phe64-Ser-Tyr-Gly-Val-Gln69 aminoacid sequence. GFP structure contains a 11-stranded β-barrel and a central helix and corresponds to a molecular weight of 26 kDa.

Immunogen

synthetic peptide corresponding amino acids 3-17 of GFP.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

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저장 등급

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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시험 성적서(COA)

Lot/Batch Number
0000425859
0000425859
0000382649
0000382649
0000350272

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특정 버전이 필요한 경우 로트 번호나 배치 번호로 특정 인증서를 찾을 수 있습니다.

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Pathogenic mechanism of recurrent mutations of SCN8A in epileptic encephalopathy
Wagnon JL, et al.
Annals of Clinical and Translational Neurology, 3(2), 114-123 (2016)
Green fluorescent protein (GFP): applications, structure, and related photophysical behavior
Zimmer M
Chemical Reviews, 102(3), 759-782 (2002)
Green fluorescent protein: a perspective
Remington SJ
Protein Science, 20(9), 1509-1519 (2011)
A phosphorylation-wide sncRNA screen reveals Protein Functional Effector sncRNAs (pfeRNAs) in human lung somatic cells
Gable T, et al.
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