H6147

HEPES

Name: HEPES
Brand: SIGMA

BioXtra, suitable for mouse embryo cell culture, ≥99.5% (titration)

동의어(들):

4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)

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크기 선택

제품정보 (DICE 배송 시 비용 별도)

실험식(Hill 표기법):

C₈H₁₈N₂O₄S

CAS 번호:

7365-45-9

Molecular Weight:

238.30

UNSPSC Code:

12161704

NACRES:

NA.75

PubChem Substance ID:

24895731

EC Number:

230-907-9

Beilstein/REAXYS Number:

883043

MDL number:

MFCD00006158

주요 문서

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제품 이름

HEPES, BioXtra, suitable for mouse embryo cell culture, ≥99.5% (titration)

InChI key

JKMHFZQWWAIEOD-UHFFFAOYSA-N

InChI

1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)

SMILES string

OCCN1CCN(CC1)CCS(O)(=O)=O

product line

BioXtra

assay

≥99.5% (titration)

form

powder

technique(s)

cell culture | embryo: suitable

pH

5.0-6.5 (25 °C, 238 g/L)

useful pH range

6.8-8.2

pKa (25 °C)

7.5
7.5

solubility

water: 500 mg/mL, clear, colorless

absorption

≤0.05 at 290 in H₂O at 33%

Quality Level

400

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Application

HEPES has been used:

As a constituent of modified Eagle′s minimum essential medium for in vitro culturing of spermatogenic cells and as a constituent of the cryoprotectant used to preserve these germ cells
As a component of modified Ham′s F-10 for the incubation of doe zygotes to carry out Haploid male hemizygous recipient production
in the preparation of 90% Percoll solution used in Percoll- gradient method for sperm collection and preparation

General description

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2 (pKa 7.55). HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg. It is typically used in cell culture at concentration between 5mM to 30mM.

Useful pH range: 6.8 - 8.2

Other Notes

Easily compare specifications for HEPES products with the HEPES specification table.

This product has been qualified for use in cell and mouse embryo culture.

저장 등급

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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문서 라이브러리 방문

Interactions of Casein and Polypeptides in Multilayer Films Studied by FTIR and Molecular Dynamics.

Lilianna Szyk-Warszyńska et al.

Polymers, 11(5) (2019-05-28)

Multilayer films containing α- and β-casein and polypeptides, poly-L-lysine (PLL), and poly-L-arginine (PLArg) were formed by the layer-by-layer technique and Fourier Transform InfraRed spectroscopy with Attenuated Total Reflection (FTIR-ATR) and FTIR/Grazing Angle analyzed their infrared spectra. We investigated the changes

Viable offspring derived from cryopreserved haploid rabbit parthenotes.

Garci'a-Ximenez F and Escriba MJ

Theriogenology, 57(4), 1319-1325 (2002)

Short-term in-vitro culture and cryopreservation of spermatogenic cells used for human in-vitro conception.

Aslam I and Fishel S

Human Reproduction, 13(3), 634-638 (1998)

The effects of L-ergothioneine and L-ascorbic acid on the in vitro maturation (IVM) and embryonic development (IVC) of sheep oocytes.

Pancarci SM, et al.

Kafkas U?niversitesi veteriner faku?ltesi dergisi, 16, 757-763 (2010)

Assessing the corrosion of biodegradable magnesium implants: a critical review of current methodologies and their limitations.

Kirkland NT, et al.

Acta Biomaterialia, 8(3), 925-936 (2012)

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문서

Embryo Culture Media

Mouse embryo media and embryo validated reagents for transgenic mouse embryo culture

HEPES

BioXtra, suitable for mouse embryo cell culture, ≥99.5% (titration)

크기 선택

제품정보 (DICE 배송 시 비용 별도)

주요 문서

속성

제품 이름

InChI key

InChI

SMILES string

product line

assay

form

technique(s)

pH

useful pH range

pKa (25 °C)

solubility

absorption

Quality Level

관련 카테고리

설명

Application

General description

Other Notes

안전성 정보

저장 등급

wgk

flash_point_f

flash_point_c

ppe

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