I4506
IgG from human serum
reagent grade, ≥95% (SDS-PAGE), essentially salt-free, lyophilized powder
동의어(들):
Human IgG
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크기 선택
제품정보 (DICE 배송 시 비용 별도)
Conjugate:
unconjugated
Technique(s):
ELISA: suitable, dot immunobinding: suitable, immunoelectrophoresis: suitable, western blot: suitable
Application:
DIBA, ELISA, IEP, WB
Citations:
228
conjugate
unconjugated
grade
reagent grade
assay
≥95% (SDS-PAGE)
form
essentially salt-free, lyophilized powder
composition
Protein, ≥90%
technique(s)
ELISA: suitable, dot immunobinding: suitable, immunoelectrophoresis: suitable, western blot: suitable
storage temp.
2-8°C
Quality Level
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General description
Purified Human IgG is isolated from pooled normal human serum by fractionation and ion-exchange chromatography. The product is supplied as an essentially salt-free (less than 1% sodium), lyophilized powder.
Application
IgG from human serum was used as a standard at a concentration of 1.274 mg/ml in ELISA to determine antibody responses to Porphyromonas gingivalis and Prevotella intermedia. It was used for immunolabeling of Vero, BHK and PtK2 cells at a concentration of 0.2 mg/ml. IgG from human serum was used as a standard in SDS-PAGE of anti-Shigella LPS antibodies.
Biochem/physiol Actions
IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
저장 등급
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Nils A Brechmann et al.
Biotechnology progress, 35(3), e2775-e2775 (2019-01-11)
High capacity magnetic protein A agarose beads, LOABeads PrtA, were used in the development of a new process for affinity purification of monoclonal antibodies (mAbs) from non-clarified CHO cell broth using a pilot-scale magnetic separator. The LOABeads had a maximum
Youhei Egami et al.
Journal of cell science, 130(24), 4168-4179 (2017-11-09)
Phagosome formation is a complicated process that requires spatiotemporally regulated actin reorganization. We found that RhoC GTPase is a critical regulator of FcγR-mediated phagocytosis in macrophages. Our live-cell imaging revealed that RhoC, but not RhoA, is recruited to phagocytic cups
Katinka Döhner et al.
Molecular biology of the cell, 13(8), 2795-2809 (2002-08-16)
After fusion of the viral envelope with the plasma membrane, herpes simplex virus type 1 (HSV1) capsids are transported along microtubules (MTs) from the cell periphery to the nucleus. The motor ATPase cytoplasmic dynein and its multisubunit cofactor dynactin mediate
Egil S Blix et al.
Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation, 21(5), 840-847 (2015-02-18)
Multiple myeloma (MM) is considered an incurable B cell malignancy, although many patients can benefit from high-dose therapy with autologous stem cell transplantation (ASCT) as a first-line treatment. In non-Hodgkin lymphoma (NHL), ASCT is usually performed after relapse with curative
Christopher Ashwood et al.
Journal of the American Society for Mass Spectrometry, 29(6), 1194-1209 (2018-04-01)
Profiling cellular protein glycosylation is challenging due to the presence of highly similar glycan structures that play diverse roles in cellular physiology. As the anomericity and the exact linkage type of a single glycosidic bond can influence glycan function, there
문서
PNGase Fast denaturing buffer and enzyme provide results similar to a conventional 20-hour protocol, reducing workflow time to about 1 hour.
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관련 콘텐츠
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Datasheet
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