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Merck

I5513

Imidazole

Molecular Biology, ≥99% (titration)

동의어(들):

3-Azapyrrole, 1,3-Diaza-2,4-cyclopentadiene, Glyoxaline

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크기 선택

제품정보 (DICE 배송 시 비용 별도)

실험식(Hill 표기법):
C3H4N2
CAS 번호:
288-32-4
Molecular Weight:
68.08
NACRES:
NA.25
PubChem Substance ID:
24896072
eCl@ss:
39161001
UNSPSC Code:
12352005
EC Number:
206-019-2
MDL number:
MFCD00005183
Beilstein/REAXYS Number:
103853

주요 문서

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제품 이름

Imidazole, Molecular Biology, ≥99% (titration)

InChI key

RAXXELZNTBOGNW-UHFFFAOYSA-N

InChI

1S/C3H4N2/c1-2-5-3-4-1/h1-3H,(H,4,5)

SMILES string

c1c[nH]cn1

grade

Molecular Biology

vapor pressure

<1 mmHg ( 20 °C)

assay

≥99% (titration)

form

crystalline powder

storage condition

dry at room temperature

technique(s)

electrophoretic mobility shift assay: suitable
molecular cloning: suitable
protein purification: suitable

color

white

pH

6.2-7.8 (25 °C)

pKa (25 °C)

6.95

bp

256 °C (lit.)

mp

88-91 °C (lit.)

solubility

water: 500 mg/mL, clear, colorless to very faintly yellow

density

1.004 g/cm3 at 20—25 °C
1.006 g/cm3 at 20—25 °C

suitability

suitable for molecular biology

application(s)

diagnostic assay manufacturing
genomic analysis
life science and biopharma
microbiology

foreign activity

DNase, RNase and protease, none detected

storage temp.

room temp

Quality Level

200

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관련 카테고리

Application

Excellent for buffers in the range of pH 6.2-7.8
Imidazole has been used

  • in the lysis, wash and elution buffer for the purification of histidine tagged Sonic Hedgehog(shh-N) protein
  • in elution buffer in stepwise gradient for the purification of histidine tagged aldo keto reductases using nickel affinity chromatography
  • as a component of homogenization buffer for the purification of phagosomal compartments from dendritic cells
  • as a binding buffer, wash buffer, and elution buffer in a study involving protein expression and purification of bioactive growth factors for use in cell culture and cellular agriculture

Biochem/physiol Actions

Imidazole derivatives have antibacterial, antifungal and anticancer functionality. It interacts with DNA and also binds to protein and stops cell division. It also acts as a microtubule destabilizing agents and inhibits topoisomerase and Cytochrome P450 Family 26 Subfamily A Member 1 (CYP26A1) enzymes. Imidazole based anticancer drug find applications in cancer chemotherapy. It is used as buffer component for purification of the histidine tagged recombinant proteins in immobilized metal-affinity chromatography (IMAC).

Features and Benefits

  • Suitable for Molecular Biology and Biochemical Research
  • Can be used as a Buffer component, for Protein separation
  • Free from DNase, NICKase, RNase, and Protease
  • Effective Buffering from pH 6.2 to 7.8 (25 °C) with a pKa of 6.9 (25 °C)

General description

Imidazole, also known as Glyoxalin, is an aromatic heterocyclic diazole and a key biological buffer with a pH range of 6.2 to 7.8 at 25°C. It has a wide range of applications in molecular biology, biochemistry, and proteomics. In proteomics, imidazole is essential for eluting histidine-tagged proteins from IMAC columns. It is a strong chelator of divalent cations, such as nickel and zinc, which disrupts the interaction between the His-tag and the metal ions. This allows for the efficient elution of purified proteins.

Furthermore, imidazole is indispensable for enhancing protein purity. It is widely used in preparing buffer solutions optimized for downstream purification columns. Imidazole solutions are also effective elution and washing agents throughout protein purification processes. Additionally, imidazole can be used for assays of horseradish peroxidase (HRP) and reverse staining of SDS-PAGE gels for protein detection. Imidazole is a versatile and valuable reagent for a wide range of biological applications. It is an essential component of many buffers and is also used in protein purification and detection protocols.

Other Notes

For additional information on our range of Biochemicals, please complete this form.

signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Eye Dam. 1 - Repr. 1B - Skin Corr. 1C

저장 등급

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 2

flash_point_f

293.0 °F - closed cup

flash_point_c

145 °C - closed cup

ppe

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges

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시험 성적서(COA)

Lot/Batch Number
0000519730
0000519729
0000519730
0000519729
0000494430

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문서 라이브러리 방문

Protein expression and purification of bioactive growth factors for use in cell culture and cellular agriculture
Semper C et al.
STAR protocols, 4, 102351-102351 (2023)
Molecular pathways regulating pro-migratory effects of Hedgehog signaling
Hochman E, et al.
The Journal of Biological Chemistry, 281(45), 33860-33870 (2006)
Imidazole: Having versatile biological activities
Verma A, et al.
Journal of Chemistry, 2013 (2013)
[Purification of proteins using polyhistidine affinity tags
Bornhorst JA and Falke JJ
Methods in Enzymology, 326, 245-254 (2000)
Isolation of Phagosomes from Dendritic Cells by Using Magnetic Beads
Manoury B
Bio-protocol, 3(14), 177-184 (2013)
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