L8918

Anti-MAP1LC3A antibody

Name: Anti-MAP1LC3A antibody
Brand: SIGMA

rabbit polyclonal

동의어(들):

Anti-Lc3, LC3 Antibody - Anti-LC3 antibody produced in rabbit, Lc3 Antibody, Anti-MAP1 light chain 3-like protein 1, Anti-MAP1A/1B light chain 3A, Anti-MAP1A/1BLC3, Anti-MAP1A/MAP1B LC3A, Anti-MAP1ALC3, Anti-MAP1BLC3, Anti-MAP1LC3A, Anti-MAP1LC3B, Anti-Microtubule-associated protein 1 light chain 3 alpha, Anti-Microtubule-associated protein 1 light chain 3 beta, Anti-Microtubule-associated proteins 1A/1B light chain 3

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제품정보 (DICE 배송 시 비용 별도)

UNSPSC Code:

12352203

NACRES:

NA.41

Conjugate:

unconjugated

Clone:

polyclonal

Application:

immunohistochemistry
immunoprecipitation (IP)
western blot

Species reactivity:

mouse, human, rat

Citations:

345

Technique(s):

immunohistochemistry: suitable
immunoprecipitation (IP): 5-10 μg using extracts of human U87 cells
western blot: 1-2 μg/mL using whole extracts of rat and mouse brain

Uniprot accession no.:

Q9H492

주요 문서

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제품 이름

Anti-LC3 antibody produced in rabbit, ~1 mg/mL, affinity isolated antibody, buffered aqueous solution

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 16-18 kDa

species reactivity

mouse, human, rat

packaging

antibody small pack of 25 μL

concentration

~1 mg/mL

technique(s)

immunohistochemistry: suitable
immunoprecipitation (IP): 5-10 μg using extracts of human U87 cells
western blot: 1-2 μg/mL using whole extracts of rat and mouse brain

UniProt accession no.

Q9H492

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

200

Gene Information

human ... MAP1LC3A(84557), MAP1LC3B(81631)
mouse ... Map1lc3a(66734), Map1lc3b(67443)
rat ... Map1lc3a(362245), Map1lc3b(64862)

Application

Anti-LC3 antibody produced in rabbit has been used in:

western blotting
immunofluorescence
immunohistochemistry
filter retardation assay
immunocytochemistry

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (3 papers)
Immunohistochemistry (1 paper)
Western Blotting (21 paper)

Biochem/physiol Actions

The gene MAP1LC3A (microtubule associated protein 1 light chain 3 α) encodes a protein that serves as a marker of autophagosomes. It is found to be expressed on surviving oligodendrocytes in Nasu-Hakola disease brains and may be involved in the induction of oligodendrogliopathy.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

The gene LC3, also referred to as MAP1LC3A (microtubule associated protein 1 light chain 3 α), is a homologue of Apg8p necessary for autophagy in yeast. The cytosolic form upon processing gets localized to the autophagosome membranes. The gene is mapped to human chromosome 20q11.22. LC3 exists in cells as cytoplasmic LC3-I (18kDa) and LC3-II (16kDa).

Immunogen

Synthetic peptide corresponding to amino acids of human LC3A isoform a, conjugated to KLH via C-terminal cysteine residue. The corresponding sequence is identical in rat and mouse.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Preparation Note

For continuous use, store at 2-8 °C for up to one month. For extended storage freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

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저장 등급

10 - Combustible liquids

wgk

WGK 3

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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시험 성적서(COA)

Lot/Batch Number

0000488229

0000492451

0000397438

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특정 버전이 필요한 경우 로트 번호나 배치 번호로 특정 인증서를 찾을 수 있습니다.

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문서 라이브러리 방문

LC3, an autophagosome marker, is expressed on oligodendrocytes in Nasu-Hakola disease brains.

Satoh J

Orphanet Journal of Rare Diseases, 9 (2014)

Endoplasmic Reticulum Stress in Heat- and Shake-Induced Injury in the Rat Small Intestine.

Peng Yin et al.

PloS one, 10(12), e0143922-e0143922 (2015-12-05)

We investigated the mechanisms underlying damage to rat small intestine in heat- and shake-induced stress. Eighteen Sprague-Dawley rats were randomly divided into a control group and a 3-day stressed group treated 2 h daily for 3 days on a rotary

Lysosomal abnormalities in hereditary spastic paraplegia types SPG15 and SPG11.

Benoît Renvoisé et al.

Annals of clinical and translational neurology, 1(6), 379-389 (2014-07-08)

Hereditary spastic paraplegias (HSPs) are among the most genetically diverse inherited neurological disorders, with over 70 disease loci identified (SPG1-71) to date. SPG15 and SPG11 are clinically similar, autosomal recessive disorders characterized by progressive spastic paraplegia along with thin corpus

TRIAD1 Is a Novel Transcriptional Target of p53 and Regulates Nutlin-3a-Induced Cell Death.

Lee J

Journal of Cellular Biochemistry (2016)

Novel anti-glioblastoma agents and therapeutic combinations identified from a collection of FDA approved drugs.

Pengfei Jiang et al.

Journal of translational medicine, 12, 13-13 (2014-01-18)

Glioblastoma (GBM) is a therapeutic challenge, associated with high mortality. More effective GBM therapeutic options are urgently needed. Hence, we screened a large multi-class drug panel comprising the NIH clinical collection (NCC) that includes 446 FDA-approved drugs, with the goal

모든 관련된 서류 보기

문서

Fluorescent Live Cell Imaging of LC3 Mediated Autophagy using LentiBrite™ Lentiviral Biosensors

High titer lentiviral particles for LC3 variants used for live cell analysis of cellular autophagy.

Anti-MAP1LC3A antibody

rabbit polyclonal

크기 선택

제품정보 (DICE 배송 시 비용 별도)

주요 문서

속성

제품 이름

biological source

conjugate

antibody form

antibody product type

clone

form

mol wt

species reactivity

packaging

concentration

technique(s)

UniProt accession no.

shipped in

storage temp.

target post-translational modification

Quality Level

Gene Information

관련 카테고리

설명

Application

Biochem/physiol Actions

Disclaimer

General description

Immunogen

Physical form

Preparation Note

안전성 정보

저장 등급

wgk

ppe

문서

시험 성적서(COA)

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