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M2128

Thiazolyl Blue Tetrazolium Bromide

Name: Thiazolyl Blue Tetrazolium Bromide
Brand: SIGMA

98%, powder

동의어(들):

3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide, MTT, Methylthiazolyldiphenyl-tetrazolium bromide

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제품정보 (DICE 배송 시 비용 별도)

실험식(Hill 표기법):

C₁₈H₁₆BrN₅S

CAS 번호:

298-93-1

Molecular Weight:

414.32

NACRES:

NA.47

PubChem Substance ID:

24896603

UNSPSC Code:

12352111

EC Number:

206-069-5

MDL number:

MFCD00011964

Beilstein/REAXYS Number:

4081397

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속성

제품 이름

Thiazolyl Blue Tetrazolium Bromide, 98%

Quality Level

200

assay

98%

form

powder

technique(s)

titration: suitable

color

dark yellow

mp

195 °C (dec.) (lit.)

solubility

2-methoxyethanol: soluble 20 mg/mL, ethanol: soluble 20 mg/mL, H₂O: 5 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

SMILES string

[Br-].Cc1nc(sc1C)-[n+]2nc(nn2-c3ccccc3)-c4ccccc4

InChI

1S/C18H16N5S.BrH/c1-13-14(2)24-18(19-13)23-21-17(15-9-5-3-6-10-15)20-22(23)16-11-7-4-8-12-16;/h3-12H,1-2H3;1H/q+1;/p-1

InChI key

AZKSAVLVSZKNRD-UHFFFAOYSA-M

설명

Application

Thiazolyl Blue Tetrazolium Blue (MTT) may be used in measurement of cell proliferation. MTT produces a yellowish solution that is converted to dark blue, water-insoluble MTT formazan by mitochondrial dehydrogenases of living cells. The blue crystals are solubilized with acidified isopropanol and the intensity is measured colorimetrically at 570 nm. MTT has been used as a histochemical/cytochemical reagent and for the detection of NAD. ADP-linked enzyme systems in tissue cannot be detected with MTT, due to binding of the cation by the cyanide trap used. MTT is rapidly reduced to the formazan, which chelates with nickel, copper, and cobalt; the cobalt chelate has been used in oxidative systems.

Thiazolyl Blue Tetrazolium Bromide has been used for the following assays:

Proliferation assay.
TNF (tumor necrosis factor) bioassay.

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pictograms

GHS08,GHS07

signalword

Warning

hcodes

H315,H319,H335,H341

pcodes

P202 - P261 - P264 - P302 + P352 - P305 + P351 + P338 - P308 + P313

Hazard Classifications

Eye Irrit. 2 - Muta. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

저장 등급

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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프로토콜

Recycling Micro-Assay of β-NAD and β-NADH

Objective: To standardize a procedure for the recycling micro-assay of β-NAD and β-NADH