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Merck

M8823

Anti-FLAG® M2 Magnetic Beads

affinity isolated antibody

동의어(들):

Monoclonal ANTI-FLAG® M2, FLAG® magnetic affinity resin, FLAG® resin for high throughput, Flag® Affinity resin, Anti-ddddk, Anti-dykddddk

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크기 선택

제품정보 (DICE 배송 시 비용 별도)

NACRES:
NA.32
UNSPSC Code:
12352203

주요 문서

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기술 서비스
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제품 이름

Anti-FLAG® M2 Magnetic Beads, affinity isolated antibody

conjugate

magnetic beads

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

M2, monoclonal

form

suspension

shelf life

2 yr at -20 °C

analyte chemical class(es)

proteins

technique(s)

affinity chromatography: suitable
immunoprecipitation (IP): suitable

bead size

20-75 μm

matrix

superparamagnetic iron impregnated 4% agarose bead, with an average diameter of 50 μm.

isotype

IgG1

capacity

≥0.6 mg/mL binding capacity

shipped in

wet ice

storage temp.

−20°C

Quality Level

200

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관련 카테고리

Application

Suitable for immunoprecipitation procedures.

Elution - FLAG® peptide, Glycine, pH 3.5, 3x FLAG® peptide

Learn more product details in our FLAG® application portal.

Disclaimer

FLAG® Affinity Gels, FLAG® tag, 3x FLAG® tag, DYKDDDDK tag

Features and Benefits

The magnetic properties allow for:
- Very rapid separation
- Significantly accelerated manipulations, such as repetitive washings
- Processing of multiple samples performed in plate formats
This leads to:
- Faster experimentation
- Better reproducibility
- More accurate quantitation of the proteins of interest

General description

Anti-FLAG M2 Magnetic Beads are 4% agarose beads bound with the Anti-FLAG M2 (mouse monoclonal) antibody. The M2 antibody recognizes the FLAG sequence at the N-terminus, Met-N-terminus and C-terminus. This alows for detection and capture of fusion proteins containing a FLAG peptide sequence.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Physical form

Supplied as a 50% suspension in 50% glycerol with 10mM sodium phosphate, 150mM sodium chloride, pH 7.4 and 0.02% (w/v) sodium azide (PBA/A).

Legal Information

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

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저장 등급

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

가장 최신 버전 중 하나를 선택하세요:

시험 성적서(COA)

Lot/Batch Number
0000514959
0000514959
0000507983
0000507983
0000507110

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특정 버전이 필요한 경우 로트 번호나 배치 번호로 특정 인증서를 찾을 수 있습니다.

COA 검색

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문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

K Kollmann et al.
Leukemia, 31(4), 934-944 (2016-10-16)
Most myeloproliferative neoplasm (MPN) patients lacking JAK2 mutations harbour somatic CALR mutations that are thought to activate cytokine signalling although the mechanism is unclear. To identify kinases important for survival of CALR-mutant cells, we developed a novel strategy (KISMET) that
Chanqiong Zhang et al.
Cancer biology & therapy, 20(9), 1213-1222 (2019-04-16)
It is verified that long non-coding RNAs (lncRNAs) play crucial roles in various cancers. LncRNA LEF1-AS1 is a reported oncogene in colorectal cancer and glioblastoma. In this study, we unveiled that LEF1-AS1 markedly increased in oral squamous cell carcinoma (OSCC)
Ephrem G Kassa et al.
PLoS pathogens, 15(6), e1007851-e1007851 (2019-06-27)
Enteropathogenic E. coli (EPEC) is an extracellular diarrheagenic human pathogen which infects the apical plasma membrane of the small intestinal enterocytes. EPEC utilizes a type III secretion system to translocate bacterial effector proteins into its epithelial hosts. This activity, which
Chao Wang et al.
Cell discovery, 2, 16006-16006 (2016-07-28)
The Hippo signaling pathway regulates tissue growth and organ size through controlling cell growth, proliferation and apoptosis. During these processes, the coactivator Yorkie partners with the transcription factor Scalloped to mediate Hippo pathway-regulated cellular functions. Here, we demonstrate that Taiman
Anastasia H Potts et al.
Nature communications, 8(1), 1596-1596 (2017-11-19)
CsrA is a post-transcriptional regulatory protein that is widely distributed among bacteria. This protein influences bacterial lifestyle decisions by binding to the 5' untranslated and/or early coding regions of mRNA targets, causing changes in translation initiation, RNA stability, and/or transcription
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문서

ANTI-FLAG® M2 Magnetic Beads

The FLAG® Expression System is a proven method to express, purify and detect recombinant fusion proteins. Sigma®, the proven provider of FLAG®, now offers a magnetic bead for immunoprecipitation, protein purification, and the study of protein-protein interactions. The ANTI-FLAG® M2 Magnetic Bead is composed of murine derived, anti-FLAG® M2 monoclonal antibody attached to superparamagnetic iron impregated 4% agarose beads, with an average diameter of 50 µm. The M2 antibody is capable of binding to fusion proteins containing a FLAG peptide sequence at the N-terminus, Met-N-terminus, or C-terminus locations in mammalian, bacterial, and plant extracts.

Anti-FLAG® M2 Magnetic Beads

Comparison of elution techniques for small-scale protein purification of FLAG® tag proteins using anti-FLAG® M2 magnetic beads.

관련 콘텐츠

Protein Expression

Protein expression technologies for various expression systems supporting research, therapeutics, and vaccine production.

Protein Purification

Protein purification techniques, reagents, and protocols for purifying recombinant proteins using methods including, ion-exchange, size-exclusion, and protein affinity chromatography.

Protein Research Guide

Find protein research tools to prepare, isolate, and analyze proteins. Organized by how to extract, protect, purify, enrich, modify, and quantify proteins.

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