T6557
Anti-γ-Tubulin Antibody
mouse monoclonal, GTU-88
동의어(들):
Anti-γ-Tubulin antibody, Mouse monoclonal
제품 이름
Anti-γ-Tubulin antibody, Mouse monoclonal, clone GTU-88, ascites fluid
biological source
mouse
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
GTU-88, monoclonal
mol wt
antigen 48 kDa
contains
15 mM sodium azide
species reactivity
rat, hamster, chicken, human, bovine, canine, Xenopus, mouse
technique(s)
immunocytochemistry: 1:5,000-1:10,000 using HeLa cells
indirect ELISA: suitable
western blot: 1:10,000 using cultured chicken fibroblast extract
isotype
IgG1
UniProt accession no.
application(s)
research pathology
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... TUBG1(7283)
mouse ... Tubg1(103733)
rat ... Tubg1(252921)
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Application
Monoclonal Anti-gamma-Tubulin is suitable for use in immunochemical applications such as immunoblotting, immunocytochemical staining of cultured cells, and in ELISA.
Monoclonal Anti-γ-Tubulin antibody has been used in western blotting, indirect immunofluorescence (IF) and immunofluorescence staining.
Monoclonal Anti-γ-Tubulin is also suitable for use in immunochemical applications such as immunoblotting, immunocytochemical staining of cultured cells and in ELISA.
Monoclonal Anti-γ-Tubulin is also suitable for use in immunochemical applications such as immunoblotting, immunocytochemical staining of cultured cells and in ELISA.
Biochem/physiol Actions
γ-Tubulin nucleates microtubule assembly throughout the mammalian cell cycle in vivo. γ-Tubulin binds microtubule minus ends and is responsible for mediating the link between microtubules and the centrosome. It functions as the microtubule nucleator at the MTOC. It binds to the β-tubulin half of the tubulin molecule, thus establishing the polarity of a microtubule, leaving the α-tubulin half exposed at the plus end. γ-Tubulin abundance is less than 1% of the level of either α- or β-tubulin. Overexpression of γ-tubulin is observed in lung cancer. The expression levels of γ-tubulin can be considered as an important prognostic indicator for patients with astrocytomas.
Monoclonal Anti-γ-Tubulin recognizes an epitope located in the N-terminal amino acids of γ-tubulin (48 kDa). Cross reactivity has been observed with human, bovine, dog, hamster, rat, mouse, chicken, and Xenopus γ-tubulin.
The antibody recognizes an epitope located within the N-terminal region of γ-tubulin.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
γ-Tubulin (48kDa) is a ubiquitous and highly conserved protein within the microtubule organizing centers (MTOCs) in eukaryotic cells. γ-Tubulin is mapped to human chromosome 17q21.2 and codes for a member of the tubulin family. Human TUBG1 transcript is widely expressed in preimplantation embryos and brain.
Monoclonal Anti-γ-Tubulin (mouse IgG1 isotype) is derived from the GTU-88 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.
Monoclonal Anti-γ-Tubulin (mouse IgG1 isotype) is derived from the GTU-88 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.
Immunogen
synthetic γ-tubulin peptide, conjugated to KLH
Physical form
The product is provided as ascites fluid with 15 mM sodium azide as a preservative.
Preparation Note
For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in "frostfree" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
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저장 등급
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Association of Aurora A and gamma-tubulin expression in astrocytomas and patient survival.
Tsai HP, et al.
Neurological Research, 36(8), 746-751 (2014)
γ-Tubulin is a centrosomal protein required for cell cycle-dependent microtubule nucleation.
Joshi HC, et al.
Nature, 356(6364), 80-80 (1992)
Human NEIL1 localizes with the centrosomes and condensed chromosomes during mitosis.
Hildrestrand GA, et al.
DNA Repair, 6(10), 1425-1433 (2007)
Gamma-tubulin is a minus end-specific microtubule binding protein.
Li Q and Joshi HC
The Journal of Cell Biology, 131(1), 207-214 (1995)
Interaction of amyotrophic lateral sclerosis (ALS)-related mutant copper-zinc superoxide dismutase with the dynein-dynactin complex contributes to inclusion formation.
Strom AL, et al.
The Journal of Biological Chemistry, 283(33), 22795-22805 (2008)
문서
Microtubules of the eukaryotic cytoskeleton are composed of a heterodimer of α- and β-tubulin. In addition to α-and β-tubulin, several other tubulins have been identified, bringing the number of distinct tubulin classes to seven.
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