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  • GABAergic pump cells of solitary tract nucleus innervate retrotrapezoid nucleus chemoreceptors. 17460107

    The retrotrapezoid nucleus (RTN) contains central respiratory chemoreceptors that are inhibited by activation of slowly adapting pulmonary stretch receptors (SARs). Here we examine whether RTN inhibition by lung inflation could be mediated by a direct projection from SAR second-order neurons (pump cells). Pump cells (n = 56 neurons, 13 rats) were recorded in the nucleus of solitary tract (NTS) of halothane-anesthetized rats with intact vagus nerves. Pump cells had discharges that coincided with lung inflation as monitored by the tracheal pressure. Their activity increased when end-expiratory pressure was raised and stopped instantly when ventilation was interrupted in expiration. Many pump cells could be antidromically activated from RTN (12/36). Nine of those were labeled with biotinamide. Of these nine cells, eight contained glutamic acid decarboxylase 67 (GAD67) mRNA and seven were found to reside in the lower half of the interstitial subnucleus of NTS (iNTS). Using the retrograde tracer cholera toxin-B, we confirmed that neurons located in or close to iNTS innervate RTN (two rats). Many such neurons contained GAD67 mRNA and a few contained glycine transporter2 (GLYT2) mRNA. Anterograde tract tracing with biotinylated dextranamide (four rats) applied to iNTS also confirmed that this region innervates RTN by a predominantly GABAergic projection. This work confirms that many rat NTS pump cells are located in and around the interstitial subnucleus at area postrema level. We demonstrate that a GABAergic subset of these pump cells innervates the RTN region. We conclude that these inhibitory neurons probably contact RTN chemoreceptors and mediate their inhibition by lung inflation.
    문서 타입:
    Reference
    카탈로그 번호:
    AB1511

  • What pump should I use with the Prep/Scale system?…

    The Prep/Scale system can be operated with a peristatic pump capable of up to 13 L/min, or a rotary lobe pump. Because the recommended tangential flow rate for all cartridges is 1 L/min up to 6 L/min, be sure that the pump chosen is capable of these rates. As a general rule, for the Prep/Scale-TFF-1 (1 ft2) cartridge, the 1.6 L/min peristaltic pump works fine. For the two larger cartridges, you may wish to use the larger 13 L/min peristaltic pump in order to operate your TFF system optimally. Too small a pump for the system, may increase the number of pump passes required to process the volume to a level that may actually degrade your final product.
    문서 타입:
    FAQ
    카탈로그 번호:
    C610

  • Sodium pump activity in the yolk syncytial layer regulates zebrafish heart tube morphogenesis. 22182522

    Na(+),K(+) ATPase pumps Na(+) out of and K(+) into the cytosol, maintaining a resting potential that is essential for the function of excitable tissues like cardiac muscle. In addition to its well-characterized physiological role in the heart, Na(+),K(+) ATPase also regulates the morphogenesis of the embryonic zebrafish heart via an as yet unknown mechanism. Here, we describe a novel non-cell autonomous function of Na(+),K(+) ATPase/Atp1a1 in the elongation of the zebrafish heart tube. Embryos lacking Atp1a1 function exhibit abnormal migration behavior of cardiac precursors, defects in the elongation of the heart tube, and a severe reduction in ECM/Fibronectin deposition around the myocardium, despite the presence of normal cell polarity and junctions in the myocardial epithelium prior to the timeframe of heart tube elongation. Interestingly, we found that Atp1a1 is not present in the myocardium at the time when cardiac morphogenesis defects first become apparent, but is expressed in an extra-embryonic tissue, the yolk syncytial layer (YSL), at earlier stages. Knockdown of Atp1a1 activity specifically in the YSL using morpholino oligonucleotides produced heart tube elongation defects like those found in atp1a1 mutants, indicating that Atp1a1 function in the YSL is necessary for heart tube elongation. Furthermore, atp1a1 expression in the YSL was regulated by the homeobox transcription factor mxtx1. Together, these data reveal a new non-cell autonomous role for Atp1a1 in cardiac morphogenesis and establish Na(+),K(+) ATPase as a major player in the genetic pathway by which the YSL regulates embryonic ECM deposition.
    문서 타입:
    Reference
    카탈로그 번호:
    05-419
    제품명:
    Anti-Myc Tag Antibody, clone 9E10

  • Which pump should I use with Steripak-GP?…

    Millipore recommends use of a pump that can generate a flow rate of approximately 100 mL / min.
    문서 타입:
    FAQ
    카탈로그 번호:
    C3236
    제품명:
    Steripak™ Filter Units