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  • What pipette or pipette tips do you recommend to recover the retentate from the Amicon Ultra concent…

    We do not have any recommendation for pipette or pipette tip in the insert-booklet of Amicon Ultra. Certain pipettes, like Eppendorf 100-1000µl, are very wide and the tip can not reach the bottom of the device. So a smaller pipette is needed. The pipette we use for all the studies is a Gilson pipetman with regular pipette tips 1-200µl from Fisher cat# 21-197-2J.
    문서 타입:
    FAQ
    카탈로그 번호:
    Multiple
    제품명:
    Multiple

  • Are ZipTip pipette tips compatible with robotic / automated sample preparation systems?…

    ZipTipm-C18 compatibility has been demonstarted with the following robotic / automated sample preparation systems:

    1. ABI Symbiot™ Sample Workstation for MALDI TOF Biospectrometry™ with Universal Cone for ZipTip (cat. no. GEN602987) and Rack Adapter for the ZipTip box.

    2. Bruker Daltonics MAP™ II and MAP™ II/8 MADLI Auto Prep Station

    3. Micromass 2700-MS and MassPREP™ Sample Preparation Stations

    4. Genomic Solutions ProMS™ Sample Preparation Station

    5. Cy-Bio CyBi™ Well 96 automated pipettor

    6. Packard BioScrences MultiPROBE

    7. Tecan GENESIS Workstation
    문서 타입:
    FAQ
    카탈로그 번호:
    C5737
    제품명:
    ZipTip® Pipette Tips

  • What is the ZipTip pipette tip material of construction?…

    The actual pipette tip is constructed of polypropylene
    문서 타입:
    FAQ
    카탈로그 번호:
    C5737
    제품명:
    ZipTip® Pipette Tips

  • How can I elute from ZipTip pipette tips directly into a nanospray needle?…

    We suggest cutting a gel loading tip (Eppendorf 0030 001 222) approximately 2-3 mm above where the tip widens from the capillary end. ZipTip binding, washing and elution are performed utilizing the standard protocols listed in the ZipTip Reversed-Phase Operating Manual. Prior to the final dispense (elution of the peptide or protein) the cut down gel loading tip is pressed on to the end of the ZipTip using a slight twisting motion. The friction fit is tight, allowing sample to be dispensed directly into an nanospray needle.

    Refer to the ZipTip Reversed-Phase Operators Manual for a detailed diagram demonstrating the above procedure.
    문서 타입:
    FAQ
    카탈로그 번호:
    C5737
    제품명:
    ZipTip® Pipette Tips

  • How are ZipTip pipette tips packaged?…

    ZipTip pipette tips are available in four pack sizes:
    • 8 Pack Resealable Tray
    • 24 Pack Resealable Tray
    • 96 Pack 96 place tip rack
    • 960 Pack 10 x 96 place tip rack
    문서 타입:
    FAQ
    카탈로그 번호:
    C5737
    제품명:
    ZipTip® Pipette Tips

  • What is a ZipTip pipette tip?…

    ZipTip pipette tips are a 10 µl pipette tip with a bed of chromatography media fixed at the end such that there is no dead volume. ZipTip is ideal for concentrating, desalting and fractionating femtomole to picomole amounts of peptide, protein or oligonucleotide samples prior to analysis.

    In operation, the pipette tip is placed onto a single or multi-channel pipette or a 22-gauge blunt-end HPLC needle. Sample is aspirated and dispensed through the tip to bind, wash and elute the analyte(s) of interest. The concentrated, purified sample is precisely eluted in 0.5-4 µL of eluant. The working volume range of ZipTip is between 1 to 100µL.

    Currently ZipTip pipette tips are available with the following resins:
    C18
    C4
    SCX
    문서 타입:
    FAQ
    카탈로그 번호:
    C5737
    제품명:
    ZipTip® Pipette Tips

  • What protocols are available for ZipTip Pipette Tips?…

    Applications work to-date has focused on sample preparation prior to MALDI-TOF mass spectrometry and direct injection electrospray/nanospray mass spectrometry.

    Protocols are available for the following:
    • Sample Preparation of Peptides or Proteins Prior to MALDI-TOF MS Using ZipTipC18, ZipTipµ-C18 and ZipTipC4 Pipette Tips (TN224) Sample Preparation of Peptides or Proteins Prior to Electrospray / Nanospray MS Using ZipTipC18, ZipTipµ-C18 and ZipTipC4 Pipette Tips (TN072)
    • Fractionation of Complex Peptide or Protein Mixtures Prior to MALDI-TOF MS Using ZipTipC18, ZipTipµ-C18 and ZipTipC4 Pipette Tips (TN226)
    • Sample Preparation of Oligonucleotides Prior to MALDI-TOF MS Using ZipTipC18, ZipTipµ-C18 Pipette Tips (TN225)
    • Enrichment of Phosphopeptides Before MALDI-TOF and Nanoelectrospray MS using ZipTipMC Pipette Tips (TN228)
    • Purification of Radiolabled Oligonucleotides using ZipTipC18 Pipette Tips (TN230) Peptide Sample
    • Preparation Prior to MALDI-TOF and Electrospray MS using ZipTipSCX Pipette Tips (TN233)
    문서 타입:
    FAQ
    카탈로그 번호:
    C5737
    제품명:
    ZipTip® Pipette Tips

  • What types of resins are available in ZipTip pipette tips?…

    ZipTip pipette tips are currently available with the following resins: C18, C4, and SCX.
    문서 타입:
    FAQ
    카탈로그 번호:
    C5737
    제품명:
    ZipTip® Pipette Tips