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Promoting strand exchange in a DNA-templated transfer reaction.

Chemical communications (Cambridge, England) (2012-12-12)
Julia Michaelis, Atsushi Maruyama, Oliver Seitz
ABSTRACT

Most DNA-templated reactions suffer from product inhibition. We explored a DNA-triggered fluorophor transfer reaction and demonstrated that comb-type polylysine-polydextran copolymers increase the turnover in template by promoting strand exchange.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Spermine, ≥99.0% (GC)
Sigma-Aldrich
Spermine, suitable for cell culture, BioReagent
Sigma-Aldrich
Spermine, ≥97%