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DR1123 Anti-GCM1 Mouse mAb (2E11)

Anti-GCM1, mouse monoclonal, clone 2E11, recognizes the ~50 kDa GCM1 in FHs 173 WE and HeLa cells and human stomach tissue. It is validated for use in ELISA, WB, ICC, and IHC on paraffin sections.

Anti-GCM1 Mouse mAb (2E11): SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.

Synonyme: Anti-Glial Cells Missing Homolog 1

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      Übersicht

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      HMMonoclonal Antibody
      Description
      OverviewRecognizes the ~50 kDa GCM1 protein in FHs 173 WE and HeLa cells and human stomach tissue.
      Catalogue NumberDR1123
      Brand Family Calbiochem®
      SynonymsAnti-Glial Cells Missing Homolog 1
      Application Data
      Detection of human GCM1 by immunoblotting. Sample: Whole cell lysate from FHs 173 WE cells. Primary antibody: Anti-GCM1 Mouse mAb (2E11) (Cat. No. DR1123) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human GCM1 by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-GCM1 Mouse mAb (2E11) (Cat. No. DR1123) (10 µg/ml). Detection: fluorescence.

      Detection of human GCM1 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human stomach tissue. Primary antibody: Anti-GCM1 Mouse mAb (2E11) (Cat. No. DR1123) (1.7 µg/ml). Detection: colorimetric.
      References
      Product Information
      Formliquid
      FormulationIn PBS, pH 7.2.
      Positive controlFHs 173 WE cells, Human stomach tissue, HeLa cells
      PreservativeNone
      Quality LevelMQ100
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Immunocytochemistry
      Paraffin Sections
      Application NotesELISA (see comments) Immunoblotting (1-5 µg/ml) Immunocytochemistry (10 µg/ml) Paraffin Sections (1.7 µg/ml)
      Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenA recombinant polypeptide corresponding to amino acids of 108-166 human GCM1, expressed as a GST fusion protein
      Clone2E11
      HostMouse
      IsotypeIgG2a
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Regulatory Review
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      DR1123 0

      Documentation

      Anti-GCM1 Mouse mAb (2E11) SDB

      Titel

      Sicherheitsdatenblatt (SDB) 

      Datenblatt

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision05-November-2010 RFH
      SynonymsAnti-Glial Cells Missing Homolog 1
      ApplicationELISA (see comments) Immunoblotting (1-5 µg/ml) Immunocytochemistry (10 µg/ml) Paraffin Sections (1.7 µg/ml)
      Application Data
      Detection of human GCM1 by immunoblotting. Sample: Whole cell lysate from FHs 173 WE cells. Primary antibody: Anti-GCM1 Mouse mAb (2E11) (Cat. No. DR1123) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human GCM1 by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-GCM1 Mouse mAb (2E11) (Cat. No. DR1123) (10 µg/ml). Detection: fluorescence.

      Detection of human GCM1 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human stomach tissue. Primary antibody: Anti-GCM1 Mouse mAb (2E11) (Cat. No. DR1123) (1.7 µg/ml). Detection: colorimetric.
      DescriptionPurified mouse monoclonal antibody. Recognizes the ~50 kDa GCM1 protein.
      BackgroundThis gene encodes a DNA-binding protein with a gcm-motif (glial cell missing motif). The encoded protein is a homolog of the Drosophila glial cells missing (GCM) gene. This protein binds to the GCM-motif (A/G)CCCGCAT, a novel sequence among known targets of DNA-binding proteins. The N-terminal DNA-binding domain confers the unique DNA-binding activity of this protein.
      HostMouse
      ImmunogenA recombinant polypeptide corresponding to amino acids of 108-166 human GCM1, expressed as a GST fusion protein
      Clone2E11
      IsotypeIgG2a
      Specieshuman
      Positive controlFHs 173 WE cells, Human stomach tissue, HeLa cells
      Formliquid
      FormulationIn PBS, pH 7.2.
      PreservativeNone
      CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Regulatory Review