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ABE2585 Anti-Mis12 Complex

Anti-Mis12 Complex Antibody, Cat. No. ABE2585, is a highly specific rabbit polyclonal antibody that targets Mis12 complex and has been tested in Immunocytochemistry, Immunofluorescence, and Western Blotting.

Anti-Mis12 Complex MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: MIS12, PMF1, DSN1, NSL1 UniProt Number: Q9H081, Q6P1K2, Q9H410, Q96IY1
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ABE2585
100 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HICC, WB, IFRbPurifiedPolyclonal Antibody
      Description
      Catalogue NumberABE2585
      DescriptionAnti-Mis12 Complex
      Background InformationMis12 complex is composed of four subunits, Protein MIS12 homolog, Polyamine-modulated factor 1, Kinetochore-associated protein DSN1 homolog, and Kinetochore-associated protein NSL1 homolog (UniProt: Q9H081, Q6P1K2, Q9H410, Q96IY1, respectively) that are encoded by genes known as MIS12 (Gene ID: 79003), PMF1 (Gene ID: 100527963), DSN1 (Gene ID: 79980), and NSL1 (also known as C1orf48, DC31, DC8, MIS14) (Gene ID: 25936) in human. The MIS12 complex is a protein interaction hub for outer kinetochore assembly. This complex acts as the primary microtubule-binding interface at kinetochores and provides a platform to recruit regulatory proteins. In human Mis12 complex subunits are shown to localize with centromere protein A (CENP-A) at inner kinetochores and internally to Ndc80 at outer kinetochores. Mis12 complex plays an essential role in chromosome segregation in vertebrates and contributes to mitotic kinetochore assembly. Reduced levels of Mis12 complex proteins are shown to result in chromosome alignment defects in both human and chicken cells, but spindle bipolarity is not disturbed. (Ref.: Kline SL et al (2006). J. Cell. Biol. 173(1): 9-17).
      References
      Product Information
      FormatPurified
      PresentationPurified rabbit polyclonal antibody in PBS without azide and with 50% glycerol.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Mis12 Complex Antibody, Cat. No. ABE2585, is a highly specific rabbit polyclonal antibody that targets Mis12 complex and has been tested in Immunocytochemistry, Immunofluorescence, and Western Blotting.
      Key Applications
      • Immunocytochemistry
      • Western Blotting
      • Immunofluorescence
      Application NotesImmunocytochemistry Analysis: A 1:1,100 dilution from a representative lot detected Mis12 complex in HeLa cells.

      Immunofluorescence Analysis: A representative lot detected Mis12 complex in HeLa cells (Rago, F., et. al. (2015). Curr Biol. 25(5):671-7).
      Biological Information
      ImmunogenFour subunits from human Mis12 complex.
      Epitopeunknown
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis polyclonal antibody detects Mis12 complex in human. It will also detect individual subunits (Mis12, PMF1, Dsn1, Nsl1) in Western Blotting.
      IsotypeIgG
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • MIS12
      • PMF1
      • DSN1
      • NSL1
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight24.10, 23.34, 40.07, and 32.16 kDa calulated for Mis12, PMF1, DSN1, and NSL1, respectively. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in recombinant Mis12 complex.

      Western Blotting Analysis: 2.2 µg/mL of this antibody detected Mis12 complex in 1 µL of recombinant Mis12 complex.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.

      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABE2585 04054839224256