Millipore Sigma Vibrant Logo

164599 Aminopeptidase N, Rat

164599
Voir les Prix & la Disponibilité

Aperçu

Replacement Information

Prix & Disponibilité

Référence DisponibilitéConditionnement Qté Prix Quantité
164599-10U
Récupération des données relatives à la disponibilité...
Disponibilité limitéeDisponibilité limitée
En stock 
Interrompu(e)
Disponible en quantités limitées
Disponibilité à confirmer
    Pour le restant : Nous vous tiendrons informé
      Pour le restant : Nous vous tiendrons informé
      Nous vous tiendrons informé
      Contacter le Service Clients
      Contact Customer Service

      Ampoule plast. 10 u
      Prix en cours de récupération
      Le prix n'a pas pu être récupéré
      La quantité minimale doit être un multiple de
      Maximum Quantity is
      À la validation de la commande Plus d'informations
      Vous avez sauvegardé ()
       
      Demander le prix
      Description
      OverviewNative aminopeptidase N purified from rat renal brush-border membranes and cleaved with papain to produce soluble enzyme. A single-chain, integral membrane protease that, functions with broad specificity to remove N-terminal amino acids from peptides. It has been reported that levels of aminopeptidase N are elevated in cancer subjects and correlate with tumor load, and thus, the enzyme is believed to play a role in angiogenesis.
      Catalogue Number164599
      Brand Family Calbiochem®
      SynonymsAPN/CD13, aminopeptidase N
      References
      ReferencesBhagwat, S.V., et al. 2003. Blood 101, 1818.
      van Hensbergen, Y., et al. 2002. Clin. Cancer Res. 8, 3747.
      Ishii, K., et al. 2001. Biol. Pharm. Bull. 24, 226.
      Product Information
      Unit of DefinitionOne unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol L-Leu-<i>p</i>NA per min at 37°C, pH 7.2
      EC number3.4.11.2
      FormLiquid
      FormulationIn 200 mM NaCl, 50 mM Tris-HCl, pH 8.0.
      Quality LevelMQ100
      Applications
      Biological Information
      Purity≥90% by SDS-PAGE
      Specific Activity≥100 units/mg protein
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw aliquot and freeze (-70°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Aminopeptidase N, Rat FDS

      Titre

      Fiche de données de sécurité des matériaux (FDS) 

      Aminopeptidase N, Rat Certificats d'analyse

      TitreNuméro de lot
      164599

      Références bibliographiques

      Aperçu de la référence bibliographique
      Bhagwat, S.V., et al. 2003. Blood 101, 1818.
      van Hensbergen, Y., et al. 2002. Clin. Cancer Res. 8, 3747.
      Ishii, K., et al. 2001. Biol. Pharm. Bull. 24, 226.
      Fiche technique

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision10-April-2008 RFH
      SynonymsAPN/CD13, aminopeptidase N
      DescriptionNative aminopeptidase N purified from rat renal brush-border membranes and cleaved with papain to produce soluble enzyme. A single-chain, integral membrane protease that functions to remove N-terminal amino acids from peptides with broad specificity. Aminopeptidase N is believed to play a role in angiogenesis and elevated levels of aminopeptidase N have been reported in patients with various types of cancer and have been correlated with tumor load. Also involved in the final stages of digestion of small peptides in the intestinal brush border.
      FormLiquid
      FormulationIn 200 mM NaCl, 50 mM Tris-HCl, pH 8.0.
      Concentration Label Please refer to vial label for lot-specific concentration
      Recommended reaction conditions200 mM Tris-HCl, pH 7.2, 100 µl substrate (L-leu-pNA; final concentration 1 mM) in 200 mM Tris-HCl, pH 7.2, enzyme sample, final volume 200 µl; incubate at 37°C for 10 min.*; cleavage is monitored at 405 nm. *Note: Incubation time of 10 min. is provided as a guideline. The incubation time depends on the activity of the enzyme and the sensitivity of the instrument and should be determined for individual assays.
      EC number3.4.11.2
      Purity≥90% by SDS-PAGE
      Specific activity≥100 units/mg protein
      Unit definitionOne unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol L-Leu-pNA per min at 37°C, pH 7.2
      Storage ≤ -70°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw aliquot and freeze (-70°C).
      Toxicity Standard Handling
      ReferencesBhagwat, S.V., et al. 2003. Blood 101, 1818.
      van Hensbergen, Y., et al. 2002. Clin. Cancer Res. 8, 3747.
      Ishii, K., et al. 2001. Biol. Pharm. Bull. 24, 226.