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544049 Pyrintegrin - Calbiochem

544049
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544049-5MG
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      Description
      OverviewA cell-permeable pyrimidine compound that promotes the adhesion of individually dissociated hESCs on matrigel- or laminin-, but not gelatin-coated surfaces and substantially reduces trypsinization-induced apoptosis (10% vs 81% TUNEL-positive cells from 2 µM Ptn or DMSO control matrigel cultures, respectively, after trypsinization), while maintaining cells’ self-renewal ability (colony formation rate = 25% or 1% 4 d after low density seeding of single-cells on matrigel surface with 2 µM Ptn or DMSO control, respectively) and the pluripotency of expanded cultures for more than 40 passages. Ptn treatment is shown to induce the activation of β1 integrin and multiple growth factor receptors, including FGFR1, IGFR1, EGFR1, and ErbB2, while blockage of β1 integrin, PI 3-K, or growth factor receptors abolishes the survival promoting effect of Ptn. Single-cell dissociation is demonstrated to hyperactivate Rho-ROCK signaling, while efficient hESC-ECM interaction suppresses Rho-Rock activation, resulting in the stabilization of newly synthesized cell surface E-cadherin, which in turn promotes cell-cell adhesion, an important determinant of hESC survival.
      Catalogue Number544049
      Brand Family Calbiochem®
      SynonymsPtn, N-(Cyclopropylmethyl)-4-(4-(6-hydroxy-3,4-dihydroquinolin-1(2H)-yl)pyrimidin-2-ylamino)benzenesulfonamide, β1 Integrin Activator
      References
      ReferencesXu, Y., et al. 2010. Proc. Natl. Acad. Sci. USA 107, 8129.
      Product Information
      FormWhite solid
      Hill FormulaC₂₃H₂₅N₅O₃S
      Chemical formulaC₂₃H₂₅N₅O₃S
      Structure formula ImageStructure formula Image
      Quality LevelMQ100
      Applications
      Biological Information
      Purity≥95% by HPLC
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Shipped at Ambient Temperature or with Blue Ice or with Dry Ice
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Protect from Light Protect from light
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
      Packaging Information
      Packaged under inert gas Packaged under inert gas
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Pyrintegrin - Calbiochem Certificats d'analyse

      TitreNuméro de lot
      544049

      Références bibliographiques

      Aperçu de la référence bibliographique
      Xu, Y., et al. 2010. Proc. Natl. Acad. Sci. USA 107, 8129.
      Fiche technique

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision31-August-2011 RFH
      SynonymsPtn, N-(Cyclopropylmethyl)-4-(4-(6-hydroxy-3,4-dihydroquinolin-1(2H)-yl)pyrimidin-2-ylamino)benzenesulfonamide, β1 Integrin Activator
      DescriptionA cell-permeable pyrimidine compound that promotes the adhesion of individually dissociated hESCs on matrigel- or laminin-, but not gelatin-coated surfaces and substantially reduces trypsinization-induced apoptosis (10% vs 81% TUNEL-positive cells from 2 µM Ptn or DMSO control matrigel cultures, respectively, after trypsinization), while maintaining cells' self-renewal ability (colony formation rate = 25% or 1% 4 d after low density seeding of single-cells on matrigel surface with 2 µM Ptn or DMSO control, respectively) and the pluripotency of expanded cultures for more than 40 passages. Ptn treatment is shown to induce the activation of β1 integrin and multiple growth factor receptors, including FGFR1, IGFR1, EGFR1, and ErbB2, while blockage of β1 integrin, PI 3-K, or growth factor receptors abolishes the survival promoting effect of Ptn. Single-cell dissociation is demonstrated to hyperactivate Rho-ROCK signaling, while efficient hESC-ECM interaction suppresses Rho-Rock activation, resulting in the stabilization of newly synthesized cell surface E-cadherin, which in turn promotes cell-cell adhesion, an important determinant of hESC survival.
      FormWhite solid
      Intert gas (Yes/No) Packaged under inert gas
      Chemical formulaC₂₃H₂₅N₅O₃S
      Structure formulaStructure formula
      Purity≥95% by HPLC
      SolubilityDMSO (100 mg/ml)
      Storage +2°C to +8°C
      Protect from light
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
      Toxicity Standard Handling
      ReferencesXu, Y., et al. 2010. Proc. Natl. Acad. Sci. USA 107, 8129.