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554715 Retro-2 - Calbiochem

554715
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Replacement Information

Tableau de caractéristiques principal

Empirical Formula
C₁₉H₁₆N₂OS

Prix & Disponibilité

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554715-25MG
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      Description
      OverviewA cell-permeable tricyclic imine compound that inhibits the endosome-to-Golgi retrograde transport, but not cellular uptake, of CtxB (Cholera toxin B chain), StxB (Shiga-like toxin B chain), and the plant toxin ricin from castor seed in HeLa cells (20 µM), without affecting cellular uptake and trafficking of Tf (Transferrin), EGF, and vesicular stomatitis virus glycoprotein VSVGts045. Retro-2 is demonstrated to selectively affect the cellular distribution of SNARE proteins Synt5 and Synt6, but not 24 other Golgi and endocytic pathway proteins. Retro-2 pretreatment is shown to effectively protect against ricin-, Stx1-, and Stx2-induced cytotoxicity in HeLa cultures in vitro (Fold of protection against 4 h toxin incubation = 2.7, 22, and 65, respectively, with 0.5 h 20 µM Retro-2 pretreatment), as well as ricin-induced (2 µg/kg by nasal spray) fatality in mice in vivo (0 mortality with one 200 mg/kg i.p. dose 1h prior to toxin exposure).
      Catalogue Number554715
      Brand Family Calbiochem®
      SynonymsCholera Toxin Retrograde Transport Blocker, Ricin Retrograde Transport Blocker, SLT Retrograde Transport Blocker, 2-(((5-Methyl-2-thienyl)methylene)amino)-N-phenylbenzamide
      References
      ReferencesStechmann, B., et al. 2010. Cell 141, 231.
      Product Information
      FormTan powder
      Hill FormulaC₁₉H₁₆N₂OS
      Chemical formulaC₁₉H₁₆N₂OS
      Structure formula ImageStructure formula Image
      Quality LevelMQ100
      Applications
      Biological Information
      Purity≥95% by HPLC
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Shipped at Ambient Temperature or with Blue Ice or with Dry Ice
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Protect from Light Protect from light
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
      Packaging Information
      Packaged under inert gas Packaged under inert gas
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Retro-2 - Calbiochem Certificats d'analyse

      TitreNuméro de lot
      554715

      Références bibliographiques

      Aperçu de la référence bibliographique
      Stechmann, B., et al. 2010. Cell 141, 231.
      Fiche technique

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision24-October-2011 RFH
      SynonymsCholera Toxin Retrograde Transport Blocker, Ricin Retrograde Transport Blocker, SLT Retrograde Transport Blocker, 2-(((5-Methyl-2-thienyl)methylene)amino)-N-phenylbenzamide
      DescriptionA cell-permeable tricyclic imine compound that inhibits the endosome-to-Golgi retrograde transport, but not cellular uptake, of CtxB (Cholera toxin B chain), StxB (Shiga-like toxin B chain), and the plant toxin ricin from castor seed in HeLa cells (20 µM), without affecting cellular uptake and trafficking of Tf (Transferrin), EGF, and vesicular stomatitis virus glycoprotein VSVGts045. Retro-2 is demonstrated to selectively affect the cellular distribution of SNARE proteins Synt5 and Synt6, but not 24 other Golgi and endocytic pathway proteins. Retro-2 pretreatment is shown to effectively protect against ricin-, Stx1-, and Stx2-induced cytotoxicity in HeLa cultures in vitro (Fold of protection against 4 h toxin incubation = 2.7, 22, and 65, respectively, with 0.5 h 20 µM Retro-2 pretreatment), as well as ricin-induced (2 µg/kg by nasal spray) fatality in mice in vivo (0 mortality with one 200 mg/kg i.p. dose 1h prior to toxin exposure).
      FormTan powder
      Intert gas (Yes/No) Packaged under inert gas
      Chemical formulaC₁₉H₁₆N₂OS
      Structure formulaStructure formula
      Purity≥95% by HPLC
      SolubilityDMSO (50 mg/ml). It is recommended to prepare a stock solution for 3 month use each time and store the unreconstituted material in solid form, protected from moisture and preferably under inert gas, for best long-term stability during storage.
      Storage +2°C to +8°C
      Protect from light
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
      Toxicity Standard Handling
      ReferencesStechmann, B., et al. 2010. Cell 141, 231.