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MAB4100

Anti-MRP1 Antibody, clone QCRL-1

clone QCRL-1, Chemicon®, from mouse

Synonym(s):

Multidrug Resistance Protein-1

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
QCRL-1, monoclonal
Species reactivity:
human
Application:
FACS, ICC, IHC (p), WB
Citations:
19
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biological source

mouse

Quality Segment

300

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

QCRL-1, monoclonal

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

flow cytometry: suitable, immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: suitable

isotype

IgG1

NCBI accession no.

NM_004996.3, NM_019862.2, NM_019898.2, NM_019899.2, NM_019900.2, NM_019901.1, NM_019902.1

UniProt accession no.

P33527

shipped in

wet ice

Gene Information

human ... ABCC1(4363)

Immunogen

Non-denatured membranes prepared from the human small cell lung cancer cell line, H69AR, which highly overexpresses MRP1 (Cole et al., 1992; Hipfner et al., 1994, 1999).

Application

Anti-MRP1 Antibody, clone QCRL-1 detects level of MRP1 & has been published & validated for use in FC, WB, IC, IH, IH(P).
Western Blot: 0.2 μg/mL, with HRP-conjugated anti mouse IgG (Hipfner et al., 1994, 1996).

Immunocytochemistry: 1:20 - 1:50 on paraformaldehyde-fixed (0.5% in PBS) or 70% methanol-fixed cytospin preparations (Hipfner et al., 1999).

Immunohistochemistry on formalin-fixed paraffini-embedded sections following antigen retrieval by microwave heating in citrate (Wright, et al., 1998).

Flow cytometry: 1:20 dilution on cells fixed in 0.5% paraformaldehyde in PBS for 30 minutes, followed by anti-mouse FITC (block and wash with solution containing 0.1% Tween) (Hipfner, et al., 1994).

Immunoprecipitation (Hipfner, et al., 1994; 1996).

Optimal working dilutions must be determined by the end user.

Biochem/physiol Actions

Reacts with a defined linear epitope (aa 918-924) of MRP1, a 190 kDa transmembrane

phosphoglycoprotein overexpressed in various human multidrug resistant tumor cell lines

and tumors (Cole et al., 1992; Hipfner et al., 1994, 1996, 1999; Wright et al, 1998). Does not cross react with the mouse mrp1, or human MDR1, MDR3,

MRP2, MRP3, MRP4, MRP5 or MRP6 gene products.

Physical form

Format: Purified
Purified immunoglobulin. Liquid in PBS with 0.1% sodium azide.

Preparation Note

Store at 2-8ºC for up to 3 months. For longer term storage, aliquot and freeze at -20ºC.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

MAB4100:

jan

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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