Immunodetection

Winning Westerns Webinar

Perhaps more than any other step in Western blotting, immunodetection conditions are subject to the greatest number of variables, in part because this step depends on antibody:antigen recognition. Our scientists know how tedious it can be to optimize immunodetection parameters; that’s why we developed the vacuum-driven SNAP i.d.^® Protein Detection System, cutting the time for blocking, probing, and washing to 30 minutes.

Go ahead, troubleshoot – and to learn more about fast immunodetection, click here.

Click on the immunodetection topics to read about the possible causes and remedies:

• Weak Signal
  
• No Signal
  
• Uneven Blot
  
• Speckled Background
  
• High Background
  
• Persistent Background
  
• High Background (Pertains to rapid immunodetection protocol only)
  
• Nonspecific Binding
  
• Reverse Images on Film (White Bands on Dark Background)
  
• Poor Detection of Small Proteins
  
• Presence of Additional Band(s) or Band(s) at the Wrong Size
  
• Large Error Bars in Quantitative Western Blotting