76307
Pefabloc® SC
Synonym(s):
4-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride, AEBSF
Sign In to View Organizational & Contract Pricing.
Select a Size
All Photos(1)
About This Item
Empirical Formula (Hill Notation):
C8H10FNO2S · HCl
CAS Number:
Molecular Weight:
239.69
UNSPSC Code:
41116107
NACRES:
NA.24
PubChem Substance ID:
MDL number:
Beilstein/REAXYS Number:
7604627
InChI
1S/C8H10FNO2S.ClH/c9-13(11,12)8-3-1-7(2-4-8)5-6-10;/h1-4H,5-6,10H2;1H
SMILES string
FS(C1=CC=C(CCN)C=C1)(=O)=O.[H]Cl
InChI key
WRDABNWSWOHGMS-UHFFFAOYSA-N
assay
≥95.0% (HPLC)
form
powder or crystals
technique(s)
HPLC: suitable
gas chromatography (GC): suitable
application(s)
forensics and toxicology
pharmaceutical (small molecule)
storage temp.
2-8°C
Quality Level
Looking for similar products? Visit Product Comparison Guide
Related Categories
Application
Pefabloc® SC may be used as an analytical standard in determining the native oligomeric state and substrate specificity of rat NTPDase1 and NTPDase2 after heterologous expression in Xenopus oocytes. It may also be used as an esterase inhibitor, which is added to the whole blood to improve the stability of ciclesonide for the determination of ciclesonide (CIC) and its active metabolite, desisobutyryl-ciclesonide (des-CIC), in human plasma using liquid chromatography-atmospheric pressure chemical ionization-tandem mass spectrometric (LC-APCI-MS/MS) method.
Recommended concentrations for use are 0.1-1 mM.
Refer to the product′s Certificate of Analysis for more information on a suitable instrument technique. Contact Technical Service for further support.
Biochem/physiol Actions
4-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride (AEBSF) is an irreversible serine protease inhibitor. Inhibition constants are similar to those of phenylmethanesulfonyl fluoride (PMSF) and diisopropylfluorophosphate (DFP). AEBSF has been shown to inhibit trypsin, chymotrypsin, plasmin kallikrein, and thrombin. As an alternative to PMSF and DFP, AEBSF offers lower toxicity, improved solubility in water, and improved stability in aqueous solutions. AEBSF can be used in cell culture in concentrations of up to 0.25 mM.
General description
Pefabloc® SC is a potent, nontoxic and relatively easy to use, irreversible serine proteinase inhibitor. It is reported to efficiently inhibit the platelet activating factor (PAF)-degrading acetylhydrolase (acetylhydrolase) in both humans and rats.
Pefabloc® SC is a trypsin inhibitor, which can be used in the synthesis of its arginine-like analogue, 4-(2-guanidinoethyl)benzenesulfonyl fluoride.
Other Notes
Potent and irreversible inhibitor of serine proteases; in contrast to PMSF, it is water soluble and non-toxic; Unaffected by albumin
Legal Information
Pefabloc is a registered trademark of Pentapharm
signalword
Danger
hcodes
Hazard Classifications
Eye Dam. 1 - Skin Corr. 1A
Storage Class
8A - Combustible corrosive hazardous materials
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Pefabloc, 4-[2-aminoethyl] benzenesulfonyl fluoride, is a new, potent nontoxic and irreversible inhibitor of PAF-degrading acetylhydrolase
Dentan C, et al.
Biochimica et Biophysica Acta, 1299(3), 353-357 (1996)
Determination of native oligomeric state and substrate specificity of rat NTPDase1 and NTPDase2 after heterologous expression in Xenopus oocytes
Failer UB, et al.
European Journal of Biochemistry, 270(8), 1802-1809 (2003)
A trypsin-based bistable switch
Postma.J.GS, et al.
Tetrahedron (2017)
Simultaneous determination of ciclesonide and its active metabolite desisobutyryl-ciclesonide in human plasma by LC?APCI-MS/MS: Application to pharmacokinetic study in healthy Chinese volunteer
Su X-M, et al.
Journal of Pharmaceutical and Biomedical Analysis, 55(1), 230-235 (2011)
V Michael Holers et al.
Frontiers in immunology, 11, 201-201 (2020-03-11)
The complement system plays an important role in the pathogenesis of rheumatoid arthritis (RA). Besides driving lectin pathway (LP) activation, the mannan-binding lectin (MBL)-associated serine proteases (MASPs) also play a key role in regulating the alternative pathway (AP). We evaluated
Related Content
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service