T4444

3, 3′,5 ,5′-Tetramethylbenzidine

Name: 3, 3′,5 ,5′-Tetramethylbenzidine
Brand: SIGMA

peroxidase substrate, chromogenic, aqueous solution

Synonym(s):

TMB

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About This Item

CAS Number:

54827-17-7

UNSPSC Code:

12352204

PubChem Substance ID:

24900209

NACRES:

NA.83

MDL number:

MFCD00007748

Product Name

3, 3′,5 ,5′-Tetramethylbenzidine Liquid Substrate, Supersensitive, for ELISA, ready to use solution

SMILES string

Cc1cc(cc(C)c1N)-c2cc(C)c(N)c(C)c2

InChI key

UAIUNKRWKOVEES-UHFFFAOYSA-N

InChI

1S/C16H20N2/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14/h5-8H,17-18H2,1-4H3

form

aqueous solution

shipped in

wet ice

storage temp.

2-8°C

Quality Level

200

Related Categories

Proteins & Enzymes

Detection Substrates & Enzymes

Application

3, 3′,5 ,5′-Tetramethylbenzidine Liquid Substrate, Supersensitive, for ELISA has been used as a substrate for peroxidase:

to determine synthesized hyaluronan and HAS2 (hyaluronan-synthesizing enzyme) expression by the transfectant
to measure the levels of α-hemolysin toxin
in imaging bound IgGs

3,3′,5,5′-Tetramethylbenzidine (TMB) is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 655 nm. The TMB reaction may be stopped with 2 M H₂SO₄ (resulting in a yellow color), and read at 450 nm.

General description

This product contains 3, 3′,5 ,5′-Tetramethylbenzidine (TMB) in a mildly acidic buffer. The substrate is supplied as a one component ready to use solution. Rate kinetics are approx. 40% faster than traditional TMB formulations. Not recommended for membrane or immunohistochemical applications that require a precipitating reaction product.

Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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