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Merck

HCCB4MAG-58K

MILLIPLEX® Human Circulating Cancer Biomarker Magnetic Bead Panel 4 - Cancer Multiplex Assay

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84
eCl@ss:
32161000

species reactivity

human

manufacturer/tradename

Milliplex®

assay range

accuracy: 80-110%
standard curve range: 0.1-100 ng/mL
(L1CAM)
standard curve range: 0.1-100 ng/mL
(Mesothelin)
standard curve range: 0.3-250 ng/mL
(Hepsin)
standard curve range: 1.4-1,000 ng/mL
(CD44)
standard curve range: 1.4-1,000 ng/mL
(Transglutaminase 2 (TGM2))
standard curve range: 13.7-10,000 pg/mL
(EpCAM)
standard curve range: 13.7-10,000 pg/mL
(Midkine)
standard curve range: 2.7-2,000 ng/mL
(ALDH1A1)
standard curve range: 20.6-15,000 pg/mL
(Carbonic Anhydrase IX (CA9))
standard curve range: 27.4-20,000 pg/mL
(Kallekrein-6)
inter-assay cv: <10%
intra-assay cv: <20%

technique(s)

multiplexing: suitable

Quality Level

200

detection method

fluorometric (Luminex® xMAP® technology)

shipped in

wet ice

Application

  • Analytes: Aldheyde dehydrogenase 1 Family, Member A1 (ALDH1A1), Carbonic Anhydrase IX (CA9), CD44, Epithelial cell adhesion molecule (EpCAM), Hepsin, Kallikrein-6, Mesothelin, Midkine, Neural cell adhesion Molecule L1 (L1CAM), Transglutaminase 2 (TGM2)
  • Recommended Sample Type: Human serum, plasma or tissue/cell/tumor culture supernatant
  • Recommended Sample Dilution: 25 μL per well of 1:5 diluted serum or plasma can be used. Tissue culture supernatant may require a dilution with an appropriate control medium prior to assay
  • Assay Run Time: Overnight (16-18 hours) at 2-8°C
  • NOTE: min DC for CA9, Midkine, Kallikrein 6 and EpCAM are in pg/mL while the rest of the analyte min DC are in ng/mL
  • Research Category: Cancer
  • Research Subcategory: Immune Response, Alzheimer′s Disease, Inflammation, Oncology, Tumor Markers

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

General description

Circulating cancer biomarkers give insight beyond intracellular oncogenesis and into how the body responds to the presence of a tumor, the dysregulation of homeostasis, and the relationship between a tumor and its environment. Multianalyte panels have become essential tools that enable researchers to measure cancer biomarkers simultaneously, teasing out these relationships and applying them to understand tumor biology.

MILLIPLEX® Human Circulating Cancer Biomarker Panel 4 is a 10-plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum and plasma samples: Aldheyde dehydrogenase 1 Family, Member A1 (ALDH1A1), Carbonic Anhydrase IX (CA9), CD44, Epithelial cell adhesion molecule (EpCAM), Hepsin, Kallikrein-6, Mesothelin, Midkine, Neural cell adhesion Molecule L1 (L1CAM), and Transglutaminase 2 (TGM2). This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.

The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Circulating Cancer
MILLIPLEX® MAP Human Circulating Cancer Biomarker Panel 4 is a Bead-Based Multiplex Assay using the Luminex xMAP technology, enabling the simultaneous analysis of multiple cancer biomarkers in human serum, plasma, and cellular tissue culture samples.

Other Notes

Please contact Technical Service for linearity of dilution.
Sensitivity: Refer to kit protocol for sensitivities of individual biomarkers.

Legal Information

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

signalword

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2

target_organs

Respiratory Tract

Storage Class

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

flash_point_f

Not applicable

flash_point_c

Not applicable

wgk

WGK 3

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Imad Tarhoni et al.
Journal of immunoassay & immunochemistry, 39(1), 84-98 (2018-01-09)
Angiogenesis is associated with tumor progression in a range of malignancies. Herein, we develop custom immunobead assays for several mechanistically important targets and evaluated these against sera from cohorts of non-small cell lung cancer (NSCLC) patients. Antigen "capture" antibodies for

Global Trade Item Number

SKUGTIN
HCCB4MAG-58K-0204055977330892
HCCB4MAG-58K-0704055977330878
HCCB4MAG-58K-0404055977330779
HCCB4MAG-58K-0804055977330861
HCCB4MAG-58K-0604055977330755
HCCB4MAG-58K-1004055977330830
HCCB4MAG-58K-0904055977330847
HCCB4MAG-58K-0304055977330786
HCCB4MAG-58K-0104055977330854
HCCB4MAG-58K-0504055977330762

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