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OP14

Anti-c-ErbB2/c-Neu (Ab-2) Mouse mAb (9G6)

liquid, clone 9G6, Calbiochem®

Sinónimos:

Anti-ErbB2, Anti-Erythroblastosis Virus, Anti-HER2, Anti-c-Neu

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NACRES:
NA.41
UNSPSC Code:
12352203
Clone:
9G6, monoclonal
Species reactivity:
human
Application:
—
Citations:
1
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biological source

mouse

Quality Segment

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

9G6, monoclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

human

manufacturer/tradename

Calbiochem®

storage condition

do not freeze

dilution

(Frozen Sections (5 µg/mL)
Immunoprecipitation (1 µg/sample)
Paraffin Sections )

isotype

IgG1

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... ERBB2(2064)

General description

Anti-c-ErbB2/c-Neu (Ab-2), mouse monoclonal, clone 9G6, recognizes the ~185 kDa c-ErbB2/c-Neu SK-BR-3 cells and breast carcinoma. It is validated for IP and IHC on frozen and paraffin sections.
Recognizes the ~185 kDa c-ErbB2/c-Neu protein in SK-BR-3 cells and breast carcinoma tissue. Does not cross-react with v-ErbB2 or the EGF receptor.
This clone expresses the kappa light chain isotype. Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with SP2/0 Ag14 myeloma cells. Recognizes the ~185 kDa c-ErbB2/c-neu protein.

Application

Frozen Sections (5 µg/mL)Immunoprecipitation (1 µg/sample)Paraffin Sections

Packaging

Please refer to vial label for lot-specific concentration.

Analysis Note

Negative Control
Normal skin tissue or HepG2 cells

Other Notes

Immunoprecipitates the 185 kDa c-neu protein and has been reported to stain formalin-fixed paraffin-embedded tissue sections of human breast carcinomas overexpressing the c-neu protein (see application references). This antibody reacts with a cell surface epitope of c-neu but does not cross-react with the EGF receptor. In one study ductal carcinomas in situ displaying large-cell, comedo growth type, were stained while none of 16 ductal carcinomas in situ of small-cell, papillary, or cribiform growth type were stained by this antibody. Antibody should be titrated for optimal results in individual systems.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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Clase de almacenamiento

10 - Combustible liquids

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Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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