C2978
CelLytic™ M
Cell Lysis Reagent, Suitable for Mammalian cell lysis and protein solubilization.
Synonym(s):
Whole-cell protein extraction solution
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About This Item
NACRES:
NA.56
UNSPSC Code:
41116134
Product Name
CelLytic™ M, Cell Lysis Reagent, Suitable for Mammalian cell lysis and protein solubilization.
form
solution
storage temp.
room temp
Quality Level
Related Categories
Analysis Note
Use 125 μl CelLytic M for 106-107 of suspended cells. For adherent cells, use 500-1,000 μL for a 100 mm plate; 200-400 μL for a 35 mm plate.
Features and Benefits
- Efficient: Up to 50% more efficient than freeze thaw, sonication and other products
- Non-denaturing: Does not interfere in downstream applications such immunoprecipitation, kinase and phosphatase assays, reporter gene assays and gel shift assays
- Convenient: Ready-to-use reagent requires no scraping from culture plates
- Fast: Rapid cell lysis at room temperature.
General description
CelLytic M is a proprietary detergent solution designed for efficient whole-cell protein extraction from cultured mammalian cells. It enables efficient and rapid cell lysis and solubilization of proteins for both suspension and adherent cells. Lysates can be used in many downstream applications without removing the CelLytic M such as reporter gene assays, Western blots/immunoprecipitation, electrophoretic mobility shift assays, phosphatase assays and kinase asssays.
Preparation Note
CelLytic M reagent efficiency for protein extraction has been tested on, but not limited to, HeLa, CHO, COS, HL-60, Jurkat, A431, PC-12, and Bovine Aorta Endothelial Cells (BAEC).
Legal Information
CelLytic is a trademark of Sigma-Aldrich Co. LLC
Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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