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07-329-AF488

Anti-acetyl-Histone H4 (Lys16) Antibody, Alexa Fluor™ 488

from rabbit, ALEXA FLUOR™ 488

Synonym(s):

Histone H4, acetyl-Histone H4 (Lys16), AlexaFluor™ 488

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
ALEXA FLUOR™ 488
Clone:
polyclonal
Application:
ICC
Citations:
1
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biological source

rabbit

Quality Segment

100

conjugate

ALEXA FLUOR™ 488

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

species reactivity

mouse, human

species reactivity (predicted by homology)

rat (based on 100% sequence homology)

technique(s)

immunocytochemistry: suitable

NCBI accession no.

NP_009563.1

UniProt accession no.

P02309

shipped in

wet ice

target post-translational modification

acetylation (Lys16)

Gene Information

human ... H4C1(8359)
mouse ... H4C1(326619)

General description

Histone H4 (UniProt P02309) is encoded by the HHF1 (ORF YBR0122; Gene ID 852294) and HHF2 (ORF N2752; Gene ID 855701) genes in yeast species. Histone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail, H4 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. Acetylation of histone H4 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs).
~10 kDa observed

Immunogen

Epitope: H4K16ac
Ovalbumin-conjugated, synthetic peptide (…GAAcKRH …) in which AcK corresponds to residue 16 of yeast histone H4.

Application

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected acetyl-Histone H4 (Lys16) in A431 and NIH/3T3 cells.
The unconjugated antibody (Cat. No. 07-329) is shown to be suitable also for ChIP, ChIP-seq, Western blotting, multiplex assay, dot blot applications.
This Anti-acetyl-Histone H4 (Lys16) Antibody, AlexaFluor 488 is validated for use in Immunocytochemistry for the detection of acetyl-Histone H4 (Lys16).

Biochem/physiol Actions

Broad species cross-reactivity is expected.
H4K16ac specificity of the unconjugated antibody (Cat. No. 07-329) has been confirmed using the Absurance Histone H3 Antibody Specificity Array (Cat. No. 16-667) and the Absurance Histone H2A, H2B, H4 Antibody Specificity Array (Cat. No. 16-665).

Analysis Note

Evaluated by Immunocytochemistry in HeLa cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected acetyl-Histone H4 (Lys16) in HeLa cells.

Other Notes

Concentration: Please refer to lot specific datasheet.

Legal Information

ALEXA FLUOR is a trademark of Life Technologies

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

07-329-AF488:

jan

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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