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SCR127 Synthetic Laminin Peptide for Rat Neural Stem Cells

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Key Applications: Cell Culture
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SCR127
5 x 3 mg  5 x 3 mg
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      Key Spec Table

      Key Applications
      CULT
      Description
      Catalogue NumberSCR127
      DescriptionSynthetic Laminin Peptide for Rat Neural Stem Cells
      OverviewLaminin, an extracellular matrix protein of the cell basement membrane, is involved in various biological activities including cell adhesion, proliferation, migration, invasion, and differentiation These heterotrimeric macromolecules - consisting of α, β, and γ chains - are difficult to extract and purify in consistently active quantities from native tissues. While some of these laminin isoforms can be isolated from either cultured cells or placental tissues, in both cases, overall protein yields tend to be relatively low and suffer from batch to batch variations. Therefore, for many stem cell scientists, it has become necessary to test each new lot laminin to ensure that the activity level of the protein was sufficient to sustain their stem cell culture.

      To overcome this time barrier, a defined synthetic laminin substrate with similar binding and functional properties of the native laminin protein is considered to be highly advantageous as it would eliminate the requirement to test and screen new lots of native protein. Millipore’s Synthetic Laminin Peptide is a suitable replacement for the native laminin protein, and has been optimized to specifically support cell adhesion, proliferation and multi-lineage differentiation of rat neural stem cells (NSCs) in vitro. Rat neural stem cells grown on tissue culture plates coated with the synthetic laminin peptides display the characteristic neural stem cell markers, Nestin and Sox-2, and furthermore possess the capacity to preferentially differentiate down both glial and neural lineages. Millipore’s Synthetic Laminin Peptide is an inexpensive, reproducible, and defined ECM substrate alternative for the in vitro culture of rat neural stem cells.

      (Note: This product has been optimized to support the growth of rat NSCs, and is not recommended for use with human or mouse NSCs).
      Materials Required but Not Delivered1. Adult Rat Hippocampal Neural Stem Cells (Catalog No. SCR022)
      2. Rat Neural Stem Cell Expansion Medium (Catalog No. SCM009)
      3. Accutase™ Cell Dissociation Solution (Catalog No. SCR005)
      4. Phosphate-Buffered Saline (1X PBS) (Catalog No. BSS-1005-B)
      5. EmbryoMax ES Cell Qualified Ultra Pure Water, sterile H20, 500 mL (Catalog No. TMS-006-B)
      6. Rodent Neuron Differentiation Kit (Catalog No. SCR035)
      7. Rodent Astrocyte Differentiation Medium (Catalog No. SCM010)
      8. Tissue culture-ware
      9. Fixative (e.g. 4% Paraformaldehyde in 1X PBS)
      10. Blocking Solution (5% normal donkey serum, 0.3% Triton X-100 in 1X PBS)
      11.Neural Stem Cell Characterization Kit (Catalog No. SCR019)
      12. Fluorescent-labeled secondary antibodies. Donkey anti-mouse IgG, Cy3 conjugated (Catalog No. AP192C), donkey anti-rabbit IgG, Cy3 conjugated (Catalog No. AP182C), donkey anti-mouse IgG, FITC conjugated (Catalog No. AP192F) and donkey anti-rabbit IgG, FITC conjugated (Catalog No. AP182F) are recommended.
      13. 4'-6-Diamidino-2-phenylindole (DAPI) / PBS solution
      14. Nunc Lab-Tek II 8 well chamber slides (Fisher Catalog No. 12-565-8)
      15. Anti-fading mounting solution
      16. Hemacytometer
      17. Microscope
      References
      Product Information
      PresentationLyophilized peptide
      Quality LevelMQ100
      Applications
      Key Applications
      • Cell Culture
      Biological Information
      Purity>95% Purity by HPLC Analysis
      Stem Cell Type
      • Neural Stem Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsThe Synthetic Laminin Peptide (Catalog No. SCR127) is shipped lyophilized and should be stored at -20°C upon receipt. Once the peptide has been reconstituted, it should be stored at 4ºC and used within 1 month.
      Packaging Information
      Material Size5 x 3 mg
      Material Package5 x 3 mg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      SCR127 04053252399190

      Related Products & Applications

      Accessories

      Catalogue Number Description  
      SCM009 Rat Neural Stem Cell Expansion Medium Show Pricing & Availability
      SCR005 Accutase cell detachment solution Show Pricing & Availability
      SCR019 Neural Stem Cell Marker Characterization Kit Show Pricing & Availability
      SCR022 Adult Rat Hippocampal Neural Stem Cells Show Pricing & Availability

      Product Families

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      ECM coated plates that meet cell type-specific needs for growth and adhesion.
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      Categories

      Life Science Research > Proteins and Enzymes > ECM Proteins for Plate Coating