Chromogenic Detection

Chromogenic detection uses enzymes to catalyze a reaction resulting in the deposition of an insoluble colored precipitate. This technique is easy to perform and requires no special equipment for analysis. However, the following should be kept in mind:

  • Sensitivity of chromogenic detection is typically at least one order of magnitude lower than with chemiluminescent reagents. 
  • Production of the precipitate can interfere with enzyme activity and limit sensitivity. 

The precipitate is difficult to strip from membrane, limiting reuse of the blot for detection of other proteins.