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IM41 Anti-TIMP-1 (Ab-2) Mouse mAb (147-6D11)

Anti-TIMP-1 (Ab-2) Mouse mAb (147-6D11) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Tissue Inhibitor of Metalloproteinase-1

IM41
  
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      Overview

      Replacement Information

      Key Spec Table

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      M
      Description
      Overview

      This product has been discontinued.

      We apologize for the inconvenience, but we do not currently have an alternative product.





      Recognizes the ~28.5 kDa TIMP-1 protein.
      Catalogue NumberIM41
      Brand Family Calbiochem®
      SynonymsAnti-Tissue Inhibitor of Metalloproteinase-1
      Application Data
      Detection of hamster TIMP-1 by immunoblotting. Sample: whole cell CHO cell lysates. Primary antibody: Anti-TIMP-1 (Ab-2) Mouse mAb (147-6D11) (Cat. No. IM41) (1 µg/ml). Detection: chemiluminescence.
      References
      ReferencesHerbst, H., et al. 1997. Am. J. Pathol. 150, 1647.
      Jung, K., et al. 1997. Int. J. Cancer 74, 220.
      Rivera, S., et al. 1997. J. Neurosci. 17, 4223.
      Vallon, R., et al. 1997. Eur. J. Biochem. 244, 81.
      Cottam, D.W. and Rees, R.C. 1993. Intl. J. Oncol. 2, 861.
      Stetler-Stevenson, W.G., et al. 1993 FASEB J. 7, 1434.
      Woessner, J.F. 1991. FASEB J. 5, 2145.
      Liotta, L.A. and Stetler-Stevenson, W.G. 1990. in Seminars in Cancer Biology, ed. M.M. Gottesman. Vol. 1(2), 99.
      Product Information
      DeclarationManufactured by Daiichi Fine Chemical Co., Ltd. Not available for sale in Japan.
      FormLiquid
      FormulationIn 100 mM sodium phosphate buffer, 0.1% BSA, pH 7.0.
      Positive controlTIMP-1 Protein (Cat. No. PF019)
      Preservative≤0.1% sodium azide
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Paraffin Sections
      Application NotesImmunoblotting (1 µg/ml)
      Paraffin Sections (5-10 µg/ml)
      Application CommentsAntibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenrecombinant, human TIMP-1
      ImmunogenHuman
      Clone147-6D11
      HostMouse
      IsotypeIgG₁
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      IM41 0

      Documentation

      Anti-TIMP-1 (Ab-2) Mouse mAb (147-6D11) Certificates of Analysis

      TitleLot Number
      IM41

      References

      Reference overview
      Herbst, H., et al. 1997. Am. J. Pathol. 150, 1647.
      Jung, K., et al. 1997. Int. J. Cancer 74, 220.
      Rivera, S., et al. 1997. J. Neurosci. 17, 4223.
      Vallon, R., et al. 1997. Eur. J. Biochem. 244, 81.
      Cottam, D.W. and Rees, R.C. 1993. Intl. J. Oncol. 2, 861.
      Stetler-Stevenson, W.G., et al. 1993 FASEB J. 7, 1434.
      Woessner, J.F. 1991. FASEB J. 5, 2145.
      Liotta, L.A. and Stetler-Stevenson, W.G. 1990. in Seminars in Cancer Biology, ed. M.M. Gottesman. Vol. 1(2), 99.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision27-August-2007 RFH
      SynonymsAnti-Tissue Inhibitor of Metalloproteinase-1
      ApplicationImmunoblotting (1 µg/ml)
      Paraffin Sections (5-10 µg/ml)
      Application Data
      Detection of hamster TIMP-1 by immunoblotting. Sample: whole cell CHO cell lysates. Primary antibody: Anti-TIMP-1 (Ab-2) Mouse mAb (147-6D11) (Cat. No. IM41) (1 µg/ml). Detection: chemiluminescence.
      DescriptionPurified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with NS1 mouse myeloma cells. Recognizes the ~28 kDa TIMP-1 protein.
      BackgroundMatrix metalloproteinases (MMPs) are a family of enzymes that are responsible for the degradation of extracellular matrix components such as collagen, laminin and proteoglycans. In addition to sequence homology, all MMPs share the following characteristics: the catalytic mechanism is dependent upon a zinc ion at the active center, they cleave one or more extracellular matrix components, they are secreted as zymogens which are activated by removal of an ~10 kDa segment from the N-terminus and their activity is regulated by endogenous inhibitors. These enzymes are involved in normal physiological processes such as embryogenesis and tissue remodeling and may play an important role in arthritis, periodontitis, and metastasis. The activation and activity of MMPs are regulated by a family of endogenous inhibitors, tissue inhibitiors of metalloproteinase (TIMP). TIMP-1 is a 28 kDa glycoprotein that is expressed by a variety of cell types. It forms a non-covalent, stoichiometric complex with both latent and active MMPs. TIMP-1 preferentially binds and inhibits MMP-9 and MMP-1 interacting through the catalytic domain of the MMP protein. TIMPs are capable of altering the metastatic potential of cancer cells and have been shown to inhibit invasion and metastasis in animal models as well as to be subject to differential expresssion in other disease states.
      HostMouse
      Immunogen speciesHuman
      Immunogenrecombinant, human TIMP-1
      Clone147-6D11
      IsotypeIgG₁
      Specieshuman
      Positive controlTIMP-1 Protein (Cat. No. PF019)
      FormLiquid
      FormulationIn 100 mM sodium phosphate buffer, 0.1% BSA, pH 7.0.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsAntibody should be titrated for optimal results in individual systems.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesHerbst, H., et al. 1997. Am. J. Pathol. 150, 1647.
      Jung, K., et al. 1997. Int. J. Cancer 74, 220.
      Rivera, S., et al. 1997. J. Neurosci. 17, 4223.
      Vallon, R., et al. 1997. Eur. J. Biochem. 244, 81.
      Cottam, D.W. and Rees, R.C. 1993. Intl. J. Oncol. 2, 861.
      Stetler-Stevenson, W.G., et al. 1993 FASEB J. 7, 1434.
      Woessner, J.F. 1991. FASEB J. 5, 2145.
      Liotta, L.A. and Stetler-Stevenson, W.G. 1990. in Seminars in Cancer Biology, ed. M.M. Gottesman. Vol. 1(2), 99.