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HCYTOMAG-60K MILLIPLEX MAP Human Cytokine/Chemokine Magnetic Bead Panel - Immunology Multiplex Assay

HCYTOMAG-60K

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Overview

Replacement Information

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This panel is available for custom premix. Talk to your Protein Specialist today!

Key Spec Table

Analytes AvailableSpecies ReactivityKey ApplicationsMultiplexing Ordering RequirementsDetection Methods
sCD40L EGF FGF-2 Flt-3 ligand Fractalkine G-CSF GM-CSF GRO IFN-α2 IFN-γ IL-1α IL-1β IL-1ra IL-2 IL-3 IL-4 IL-5 IL-6 IL-7 IL-8 IL-9 IL-10 IL-12 (p40) IL-12 (p70) IL-13 IL-15 IL-17A IP-10 MCP-1 MCP-3 MDC (CCL22) MIP-1α MIP-1β PDGF-AB/BB RANTES TGF-α TNF-α TNF-β VEGF Eotaxin/CCL11 PDGF-AA HMplexPlease note: Sample Type must be selected when configuring this kit. RANTES, PDGF-AA and PDGF-AB/AA cannot be plexed with other analytes for serum/plasma.Luminex xMAP
Description
Catalogue NumberHCYTOMAG-60K
Trade Name
  • MILLIPLEX
DescriptionMILLIPLEX MAP Human Cytokine/Chemokine Magnetic Bead Panel - Immunology Multiplex Assay
OverviewTo identify specific cytokines involved in any inflammatory or immune response, it might be necessary to screen panels of cytokines, often requiring some level of automation and/or high throughput. Magnetic Beads can make the process of automation and high throughput screening easier with features such as walk-away washing.

The MILLIPLEX® MAP Human Cytokine / Chemokine panel enables you to focus on the therapeutic potential of cytokines as well as the modulation of cytokine expression. Coupled with the Luminex® xMAP® platform in a magnetic bead format, you receive the advantage of ideal speed and sensitivity, allowing quantitative multiplex detection of dozens of analytes simultaneously, which can dramatically improve productivity.

References:
Mol Psychiatry. 2016 May 24. doi: 10.1038/mp.2016.77.
Autism with intellectual disability is associated with increased levels of maternal cytokines and chemokines during gestation.
Jones KL, Croen LA, Yoshida CK, Heuer L, Hansen R, Zerbo O, DeLorenze GN, Kharrazi M, Yolken R, Ashwood P, Van de Water J.

Aust N Z J Psychiatry. 2016 Jun 16. pii: 0004867416652736.
Inflammatory cytokines in major depressive disorder: A case-control study.
Cassano P, Bui E, Rogers AH, Walton ZE, Ross R, Zeng M, Nadal-Vicens M, Mischoulon D, Baker AW, Keshaviah A, Worthington J, Hoge EA, Alpert J, Fava M, Wong KK, Simon NM.

PLoS One. 2016 Feb 26;11(2):e0149411. doi: 10.1371/journal.pone.0149411. eCollection 2016.
B Cell, Th17, and Neutrophil Related Cerebrospinal Fluid Cytokine/Chemokines Are Elevated in MOG Antibody Associated Demyelination.
Kothur K, Wienholt L, Tantsis EM, Earl J, Bandodkar S, Prelog K, Tea F, Ramanathan S, Brilot F, Dale RC.

Transl Psychiatry. 2016 May 31;6(5):e822. doi: 10.1038/tp.2016.91.
Prenatal immune programming of the sex-dependent risk for major depression.
Gilman SE, Cherkerzian S, Buka SL, Hahn J, Hornig M, Goldstein JM.
Background Information“Cytokine” is a general term used for a diverse group of soluble proteins and peptides which act as regulators under both normal and pathological conditions to modulate the functional activities of individual cells and tissues. These proteins also mediate direct interactions between cells and regulate processes taking place in the extracellular environment. Cytokines differ from hormones in that they act on a wider spectrum of target cells. Also, unlike hormones, they are not produced by specialized cells which are organized in specialized glands. The cytokine group of proteins includes lymphokines, interferons, colony stimulating factors and chemokines. Cytokine and chemokine research plays a significant role in achieving a deeper understanding of the immune system and its multi-faceted response to most antigens, as well as disease states such as inflammatory disease, allergic reactions, irritable bowel disease (IBD), sepsis, and cancer.
References
Product Information
Detection methodLuminex xMAP
ConfigurationDesign your multiplex kit by choosing available analytes within this panel.
Precision, %
  • Intra-assay: 2 - 13%
  • Inter-assay: 5 - 19%
Multiplex Ordering RequirementsPlease note: Sample Type must be selected when configuring this kit. RANTES, PDGF-AA and PDGF-AB/AA cannot be plexed with other analytes for serum/plasma.
Panel TypeMAGNETIC Cytokines/Chemokines
Quality LevelMQ200
Applications
ApplicationSimultaneously analyze multiple cytokine and chemokine biomarkers with Bead-Based Multiplex Assays using the Luminex technology, in human serum, plasma and cell culture samples.
Key Applications
  • Multiplexing
Application Notes• This is an overnight or two-hour incubation assay; however, for best results an overnight incubation is recommended.

• This assay requires 25 µl of neat plasma or serum, or 25 μL cell culture supernatant per well.

• This kit may be used for the analysis of all or any combination of the cytokines and chemokines in serum, plasma or tissue/cell lysate and culture supernatant samples with the exception of RANTES, PDGF-AA and PDGF-AB/BB.

• RANTES, PDGF-AA and PDGF-AB/BB cannot be plexed with other cytokines in this panel due to a required 1:100 dilution of plasma/serum samples.
Biological Information
Species Reactivity
  • Human
Analytes Available
  • sCD40L
  • EGF
  • FGF-2
  • Flt-3 ligand
  • Fractalkine
  • G-CSF
  • GM-CSF
  • GRO
  • IFN-α2
  • IFN-γ
  • IL-1α
  • IL-1β
  • IL-1ra
  • IL-2
  • IL-3
  • IL-4
  • IL-5
  • IL-6
  • IL-7
  • IL-8
  • IL-9
  • IL-10
  • IL-12 (p40)
  • IL-12 (p70)
  • IL-13
  • IL-15
  • IL-17A
  • IP-10
  • MCP-1
  • MCP-3
  • MDC (CCL22)
  • MIP-1α
  • MIP-1β
  • PDGF-AB/BB
  • RANTES
  • TGF-α
  • TNF-α
  • TNF-β
  • VEGF
  • Eotaxin/CCL11
  • PDGF-AA
Cross ReactivityCross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Accuracy
  • 87% - 107%
Physicochemical Information
Sensitivity
  • Refer to kit protocol for sensitivities for individual cytokines/chemokines.
Standard Curve Range
  • 3.2–10,000 pg/mL
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage for kit components is 2 - 8°C.
Packaging Information
Material Size96-well plate
Material Package96 well plate
Transport Information
Supplemental Information
Specifications

Documentation

Protocols

Title
Protocol- HCYTOMAG-60K
Protocol: Human Cytokine / Chemokine Magnetic Bead Panel

MILLIPLEX MAP Human Cytokine/Chemokine Magnetic Bead Panel - Immunology Multiplex Assay SDS

Title

Safety Data Sheet (SDS) 

Brochure

Title
A closer look at immunoassay platforms. Beyond fit for purpse. Bring your biomarkers to life with Immunoassay Platform Solutions.
Custom Immunoassay Menu of Services
MAGPIX®and Luminex 200™ - Multiplex Detection of Inflammation Pathway Analytes
The Power of Biomarker Analysis

Technical Info

Title
96 well Template 2 Up
96 well Template Single
HCYTOMAG-60K xPonent Protocol Template Version 3.1
HCYTOMAG-60K xPonent Protocol Template Version Magpix
HCYTOMAG-60K xPonent Standard Template Version 3.1
HCYTOMAG-60K xPonent Standard Template Version Magpix
Quality Control Range Sheet- HCC 121A and HCC 221A
Quality Control Range Sheet- HCY-116 and HCY-216
Quality Control Ranges: Milliplex Human Cytokine / Chemokine Immunoassay

Data Sheet

Title
Full Service Custom-built Assays

Posters

Title
Inflammation in Neurodegenerative Diseases
MILLIPLEX MAP: Exercise, Signaling, and Your Brain
MILLIPLEX®MAP Cell Signaling Wall Chart