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Merck

CP45

Anti-Clathrin Heavy Chain Mouse mAb (X22)

liquid, clone X22, Calbiochem®

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NACRES:
NA.43
UNSPSC Code:
12352203
Clone:
X22, monoclonal
Species reactivity:
bovine, mouse, rat, human
Application:
Citations:
5
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biological source

mouse

Quality Segment

antibody form

purified antibody

antibody product type

primary antibodies

clone

X22, monoclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

bovine, mouse, rat, human

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

dilution

(Immunoblotting (1:500)
Immunofluorescence (1:1000, indirect)
Immunoprecipitation )

isotype

IgG1

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... CLTCL1(8218)

General description

Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with Sp2/0-Ag14 mouse myeloma cells. Recognizes the ~180 kDa clathrin heavy chain protein.
Recognizes the ~180 kDa clathrin heavy chain protein.
This Anti-Clathrin Heavy Chain Mouse mAb (X22) is validated for use in Immunoblotting, Immunofluorescence, Immunoprecipitation for the detection of Clathrin Heavy Chain.

Immunogen

Human
clathrin heavy chain purified from human brain

Application

Immunoblotting (1:500)

Immunofluorescence (1:1000, indirect)

Immunoprecipitation (see comments)

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In PBS.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C).

Other Notes

Bauerfeind, R., et al. 1996. J. Neurocytol.25, 701.
Zhang, J.Z., et al. 1994. Cell78, 751.
Herskovits, J.S., et al. 1993. J. Cell Biol.122, 565.
Van Der Bliek, A.M., et al. 1993. J. Cell Biol.122, 553.
Pearse, B.M. and Robinson, M.S. 1990. Ann. Rev. Cell Biol.6, 151.
Brodsky, F.M. 1985. J. Cell Biol.101, 2047.
Immunoprecipitation of triskelions allows both the clathrin heavy chain and the associated light chain polypeptides to be examined. Antibody should be titrated for optimal results in individual systems.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)


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Clase de almacenamiento

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable



Certificados de análisis (COA)

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