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Merck

B4429

BIS-TRIS

BioPerformance Certified, suitable for cell culture, suitable for insect cell culture, ≥98.0%

Sinónimos:

2,2-bis(hidroximetil)-2,2′,2″-nitrilotrietanol, 2-Bis(2-hidroxietil)amino-2-(hidroximetil)-1,3-propanodiol, Bis(2-hidroxietil)amino-tris(hidroximetil)metano

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About This Item

Fórmula empírica (notación de Hill):
C8H19NO5
Número CAS:
6976-37-0
Peso molecular:
209.24
UNSPSC Code:
12161700
NACRES:
NA.25
PubChem Substance ID:
24891775
EC Number:
230-237-7
Beilstein/REAXYS Number:
2205275
MDL number:
MFCD00002853

Nombre del producto

BIS-TRIS, BioPerformance Certified, suitable for cell culture, suitable for insect cell culture, ≥98.0%

InChI key

OWMVSZAMULFTJU-UHFFFAOYSA-N

InChI

1S/C8H19NO5/c10-3-1-9(2-4-11)8(5-12,6-13)7-14/h10-14H,1-7H2

SMILES string

OCCN(CCO)C(CO)(CO)CO

grade

BioPerformance Certified

assay

≥98.0%

form

crystalline powder

technique(s)

cell culture | insect: suitable
cell culture | mammalian: suitable

impurities

endotoxin and total aerobic microbial count, tested

useful pH range

5.8-7.2

pKa (25 °C)

6.5

mp

104 °C

solubility

H2O: 500 mg/mL, clear, colorless to very faintly yellow

anion traces

≤5 ppm

suitability

suitable for supravital staining of living blood cells

application(s)

diagnostic assay manufacturing

Quality Level

400

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Categorías relacionadas

Application


  • A new test for the laboratory diagnosis of spherocytosis: This study introduces a novel diagnostic assay utilizing BIS-TRIS buffer, aimed at improving the detection and characterization of spherocytosis in clinical settings. The buffer′s stability and optimal pH make it suitable for routine use in clinical laboratories, contributing to accurate and reproducible diagnostic results (Vettore et al., 1984).

Other Notes

Easily compare specifications for BIS-TRIS products with the BIS-TRIS specification table.

General description

Bis-Tris is a zwitterionic buffer that is used in biochemistry and molecular biology research. It is structurally analogous to the the Good buffers that were developed to provide buffers in the pH range of 6.15 - 8.35 for wide applicability to biochemical studies. The useful pH range of Bis-Tris is 5.8 - 7.2.

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H319

Hazard Classifications

Eye Irrit. 2

Clase de almacenamiento

11 - Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Certificados de análisis (COA)

Lot/Batch Number
0000514133
0000514144
0000514142
0000514144
0000514142

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Micro RNAs (miRNAs) have been shown to circulate in biological fluids and are enclosed in vesicles such as exosomes; they are present in urine and represent a noninvasive methodology to detect biomarkers for diagnostic testing. The low abundance of RNA
Alastair W Skeffington et al.
Plant physiology, 165(2), 866-879 (2014-05-02)
The first step on the pathway of starch degradation in Arabidopsis (Arabidopsis thaliana) leaves at night is the phosphorylation of starch polymers, catalyzed by glucan, water dikinase (GWD). It has been suggested that GWD is important for the control of
Zahra Sabouri et al.
Proceedings of the National Academy of Sciences of the United States of America, 111(25), E2567-E2575 (2014-05-14)
The best-understood mechanisms for achieving antibody self/non-self discrimination discard self-reactive antibodies before they can be tested for binding microbial antigens, potentially creating holes in the repertoire. Here we provide evidence for a complementary mechanism: retaining autoantibodies in the repertoire displayed
X Chen et al.
Oncogene, 33(39), 4724-4734 (2013-10-22)
Uveal melanoma (UM) is a genetically and biologically distinct type of melanoma, and once metastatic there is no effective treatment currently available. Eighty percent of UMs harbor mutations in the Gαq family members GNAQ and GNA11. Understanding the effector pathways
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