Gel electrophoresis

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Geles de preparación manual para PAGE y SDS-PAGE utilizando los kits de moldeo de gel bis-Tris mPAGE® TurboMix

Resumen de la química del gel de poliacrilamida e instrucciones detalladas para preparar a mano geles de poliacrilamida con los kits de moldeo del gel bis-Tris TurboMix mPAGE®

Introducción a la SDS-PAGE - Separación de proteínas basada en el tamaño

Introducción a la PAGE Aprenda sobre el fondo y el protocolo de la SDS-PAGE para la separación de proteínas en función del tamaño en un gel de poliacrilamida.

Electroforesis en gel

Los métodos de electroforesis en gel de proteínas se utilizan para la separación de proteínas en preparación para aplicaciones posteriores como la inmunoelectrotransferencia y la espectrometría de masas.

Dispositivo de electroforesis bidimensional en gel automatizada Auto2D®

El dispositivo de electroforesis 2-D Auto2D® automatiza por completo los métodos de electroforesis bidimensional difíciles de una manera rápida, fácil y reproducible. Localice proteínas difíciles de encontrar utilizando el sistema Auto2D® en menos de dos horas con una gran reproducibilidad.

Tampones de migración TAE y TBE, receta y vídeo

EL TAE y el TBE se utilizan como tampones de migración para la electroforesis de ácidos nucleicos, pero tienen algunas diferencias importantes. Revise nuestras recetas y vea el video para darle a su aplicación la mayor oportunidad de éxito.

Sistema de purificación de agua de laboratorio Milli-Q^® IX

Agua purificada de tipo II desde el grifo. Los sistemas Milli-Q® IX son compactos, ofrecen 4 caudales, 3 tamaños de depósito y supervisión de la calidad directamente en la interfaz de la pantalla táctil del dispensador.

Sistema mPAGE^® Lux Casting para geles de SDS-PAGE

Descubra el sistema mPAGE® Lux Casting para geles de SDS-PAGE listos para usar en 3 minutos. Geles SDS-PAGE recientes que están listos cuando los necesita.

Protein Visualization

Prior to probing a membrane using precious antibodies, it is helpful to visualize the transferred proteins on the blot to ensure complete transfer and even loading. This page describes possible causes and potential remedies for challenges encountered during protein visualization.

DNA / Protein Electrophoresis and Troubleshooting Tables

Fluorescent Multiplex Detection using Antibody Atto Dye Conjugates

Immunoblotting (Western blot transfer) is a common technique in modern proteomics research.

Precipitation Procedures

This page describes protein precipitation as an optional step in sample preparation for 2-D electrophoresis with Cytiva products.

Performing a Purity and Homogeneity Check

This page shows how to perform a purification and homogeneity check of membrane proteins with products from Cytiva.

Troubleshooting first-dimension IEF: Ettan™ IPGphor™ 3 Isoelectric Focusing System

Troubleshooting first-dimension isoelectric focusing with Ettan IPGphor 3 Isoelectric Focusing System from Cytiva.

Preparing for IEF

This protocol shows how to prepare the Immobiline® DryStrip Kit and electrodes strips.

Reproducibility with Biological Buffers

Biological buffers are organic substances that maintain a constant pH over a given range by neutralizing the effects of hydrogen ions.

Formamide Molecular Biology Reagent

Formamide is a reagent that is an ionizing solvent in aqueous buffers. It is widely utilized in biochemistry and molecular biology, particularly in nucleic acids research.

Paper-Bridge Loading

This page shows how to apply higher sample volumes and protein amounts with paper bridges in isoelectric focusing with Cytiva products

Applying Equilibrated Immobiline® DryStrip Gels

This page describes how to apply equilibrated Immobiline® DryStrip gels on the ExcelGel SDS gels.

Detection of GST-tagged Proteins with Western Blot

This page describes how to monitor expression and purification of GST-tagged proteins by Western blot analysis using Cytiva products.

Proteomic analysis of membrane proteins

This page shows how to perform a proteomic analysis of membrane proteins to identify and quantitate all proteins expressed in a cell or a tissue with products from Cytiva.

Auto2D® 2-D Gel Electrophoresis Device Frequently Asked Questions

Auto2D® 2-D Gel Electrophoresis Device frequently asked questions for 2-D PAGE and additional 2DE protein separation workflow needs.

How to Run an mPAGE™ Protein Gel Using a Bio-Rad Electrophoresis System

Instructions for setting up an mPAGE SDS-PAGE precast protein gel for gel electrophoresis using a Bio-Rad Mini-PROTEAN® electrophoresis system.

Winning Westerns Technique Talk: Perfecting Western Blotting

In this webinar, Alli Lynch, Ph.D. reviews the basics of Western blotting, identifies common troubleshooting issues, and provides tips for Western blotting success.

Precast Agarose Gels for RNA Electrophoresis

Precast Agarose Gels for RNA Electrophoresis are suitable for separating RNA sizes from 0.25 to 10 kb.

Auto2D® Automated 2-D Gel Electrophoresis Device Protocol

The Auto2D® Automated 2-D Gel Electrophoresis Device video and detailed protocol provide reliable methods to prepare your samples and operate the Auto2D® Device.

ReadyBlue Protein Gel Staining Protocols

ReadyBlue™ Protein Gel Stain offers rapid Coomassie® staining for polyacrylamide gels with a simplified protocol.

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

Two-Dimensional Separation of Protein Samples

This page describes two-dimensional separation of protein samples with Cytiva products.

Troubleshooting 2-D DIGE Results

Troubleshooting table for 2D DIGE results lists problems, causes, and prevention methods for future experiments.

Hand-casting gels for PAGE and SDS-PAGE using mPAGE® TurboMix Bis-Tris Gel Casting Kits

Simplify your SDS-PAGE gel preparation with our pre-mixed solutions and protocols to achieve precise protein separation.

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