Development of a headspace solid-phase microextraction procedure for the determination of free volatile fatty acids in waste waters.

Development of a headspace solid-phase microextraction procedure for the determination of free volatile fatty acids in waste waters.

Journal of chromatography. A (2000-04-11)

M Abalos, J M Bayon, J Pawliszyn

RESUMEN

An analytical procedure based on headspace solid-phase microextraction (SPME) coupled to GC-flame ionization detection/Negative Chemical Ionization Mass Spectrometry has been developed for the determination of free volatile fatty acids (C2-C7) in waste water samples. Five different coatings have been evaluated and polydimethylsiloxane-Carboxen was the only fiber that allows a successful extraction of the shortest chain fatty acids (acetic and propionic). Several parameters such as extraction time and temperature, desorption conditions, agitation speed and sample volume have been optimized using the polydimethylsiloxane-Carboxen fiber. The linear dynamic range was over two-four orders of magnitude, depending on the acid. Procedural detection limits were in the low to medium microg/l levels and the RSDs were between 5.6% and 13.3%. To evaluate the applicability of the developed SPME procedure on real samples, fermented urban wastewaters were analysed.

MATERIALES

Número de producto

Marca

Descripción del producto

57298-U

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Montaje de fibra SPME, 50/30µm DVB/CAR/PDMS, StableFlex (1 cm), needle size 23 ga, Autosampler, pk of 3, plain gray hub

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Montaje de fibra SPME, 50/30µm DVB/CAR/PDMS, StableFlex (1cm), needle size 24 ga, Manual Holder, pk of 3, plain gray hub

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57329-U

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Montaje de fibra SPME, 50/30µm DVB/CAR/PDMS, StableFlex (1 cm), needle size 24 ga, Autosampler, pk of 3, plain gray hub

20358

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ORBO^™ 90 Carboxen^® 564 (20/45) 160/80 mg, W,F,F separators, O.D. × L 6 mm × 75 mm, pkg of 25 ea, for analyte group MEK (methylethyl ketone)

20355

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25467

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57318

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25461

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Columna capilar para GC Carboxen^®-1006 PLOT, L × I.D. 30 m × 0.53 mm, average thickness 30 μm

57319

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Fibra de carboxeno/polidimetilsiloxano (DVB/CAR/PDMS) de SPME, 85 μm CAR/PDMS, StableFlex (1 cm), needle size 23 ga, Autosampler, pk of 3, light blue hub

24246

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Fibra de carboxeno/polidimetilsiloxano (DVB/CAR/PDMS) de SPME, 85 μm CAR/PDMS, StableFlex (1 cm), needle size 24 ga, Autosampler, pk of 3, light blue hub

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Tubo absorbente ORBO^™-93, ORBO tube I.D. × L 6 mm × 95 mm, Bed A 180 mg, Bed B 90 mg, 60/80 mesh, pkg of 25 ea

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28718-U

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Tenax^® TA / Carboxen^® 1018, glass TD tube, preconditioned, O.D. × L 1/4 in. × 3 1/2 in., Sealed with (Swagelok^® End-Fittings), pkg of 10 ea

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