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EZGLP1T-36K Multi Species GLP-1 Total ELISA, EZGLP1T-36K

EZGLP1T-36K
96-well plate  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Analytes AvailableSpecies ReactivityKey Applications
Glucagon Like Peptide-1 H, M, RELISA
Description
Catalogue NumberEZGLP1T-36K
DescriptionMulti Species GLP-1 Total ELISA, EZGLP1T-36K
Background InformationGlucagon-like peptide-1 (GLP-1) is a 30 amino acid gut hormone secreted from the intestinal L-cells. The GLP-1 sequence is highly conserved among mammals and is released in its active form (7-36) in response to food intake. It is quickly degraded into its inactive form (9-36) after cleavage by DPPIV. GLP-1 reduces glucose levels by regulating pancreatic secretion, slowing gastric emptying and lowering the desire for food intake. Therefore, it is an important biomarker for the study of diabetes and obesity. This kit measures both the active (7-36) and inactive (9-36) forms of GLP-1.
References
Product Information
Precision, %
  • Intra-assay: < 5%
  • Inter-assay:< 12%
Quality LevelMQ100
Applications
ApplicationThis Multi Species GLP-1 Total ELISA, EZGLP1T-36K is used to measure & quantify Glucagon Like Peptide-1 levels in Metabolism research.
Key Applications
  • ELISA
Application NotesThis is a 3.5-hour assay at room temperature requiring 50 uL of serum, plasma or culture samples per well.
Biological Information
SpecificityThe antibody pair used in this assay is specific to GLP-1 (7-36) and (9-36) and has no cross-reactivity with GLP-2, GIP, Glucagon or Oxyntomodulin.
Species Reactivity
  • Human
  • Mouse
  • Rat
Analytes Available
  • Glucagon Like Peptide-1
Cross ReactivityGLP-1 is conserved in almost all mammals.
Entrez Gene Number
Entrez Gene SummaryThe protein encoded by this gene is actually a preproprotein that is cleaved into four distinct mature peptides. One of these, glucagon, is a pancreatic hormone that counteracts the glucose-lowering action of insulin by stimulating glycogenolysis and gluconeogenesis. Glucagon is a ligand for a specific G-protein linked receptor whose signalling pathway controls cell proliferation. Two of the other peptides are secreted from gut endocrine cells and promote nutrient absorption through distinct mechanisms. Finally, the fourth peptide is similar to glicentin, an active enteroglucagon.
Gene Symbol
  • GCG
  • GRPP
  • GLP1
  • GLP2
  • glucagon
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P01275 # Glicentin may modulate gastric acid secretion and the gastro-pyloro-duodenal activity. May play an important role in intestinal mucosal growth in the early period of life.
SIZE: 180 amino acids; 20909 Da
SUBCELLULAR LOCATION: Secreted.
TISSUE SPECIFICITY: Glucagon is secreted in the A cells of the islets of Langerhans. GLP-1, GLP-2, oxyntomodulin and glicentin are secreted from enteroendocrine cells throughout the gastrointestinal tract. GLP1 and GLP2 are also secreted in selected neurons in the brain.
PTM: Proglucagon is post-translationally processed in a tissue- specific manner in pancreatic A cells and intestinal L cells. In pancreatic A cells, the major bioactive hormone is glucagon cleaved by PCSK2/PC2. In the intestinal L cells PCSK1/PC1 liberates GLP-1, GLP-2, glicentin and oxyntomodulin. GLP-1 is further N-terminally truncated by post-translational processing in the intestinal L cells resulting in GLP-1(7-37) GLP-1-(7-36)amide. The C-terminal amidation is neither important for the metabolism of GLP-1 nor for its effects on the endocrine pancreas.
SIMILARITY: SwissProt: P01275 ## Belongs to the glucagon family.
MISCELLANEOUS: In the glucagon antagonist, His-53 and Phe-58 are missing. This antagonist has been successfully utilized to reduce glucose concentration in vivo.
Accuracy
  • Recovery: 90 - 110%
  • Linearity 90 - 132%
Physicochemical Information
Sensitivity
  • MinDC is 1.5 pM
Standard Curve Range
  • 4.1 – 1000 pM
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • For research use only. Not for use in diagnostic procedures.
Storage and Shipping Information
Packaging Information
Material Size96-well plate
Transport Information
Supplemental Information
Specifications

Documentation

Protocols

Title
Protocol: GLP-1 Total ELISA

Multi Species GLP-1 Total ELISA, EZGLP1T-36K SDS

Title

Safety Data Sheet (SDS) 

Brochure

Title
A closer look at immunoassay platforms. Beyond fit for purpse. Bring your biomarkers to life with Merck Millipore Immunoassay Platform Solutions.
Custom Immunoassay Menu of Services

Technical Info

Title
96 well Template 2 Up
96 well Template Single
Quality Control Ranges - GLP-1 TOTAL ELISA

Data Sheet

Title
Full Service Custom-built Assays (Merck)