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203729 (+)-Brefeldin A, Eupenicillium brefeldianum - CAS 20350-15-6 - Calbiochem

203729
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Overview

Replacement Information

Key Spec Table

Empirical FormulaCAS #
C₁₆H₂₄O₄20350-15-6

Products

Catalogue NumberPackaging Qty/Pack
203729-1MG Plastic ampoule 1 mg
Description
OverviewSpecifically and reversibly blocks translocation of proteins from the endoplasmic reticulum (ER) to the Golgi apparatus without affecting endocytosis or lysosome function. Inhibitor of HDL-mediated cholesterol efflux. Causes disassembly of the Golgi complex and ER swelling in a variety of mammalian cell lines at <40 ng/ml. Blocks binding of ADP-ribosylation factor to the Golgi and inhibits the GDP-GTP exchange. Inhibits the activity of BIG1 and BIG2, the guanine-nucleotide exchange proteins for ADP-ribosylation factors. Also available as a 25 mM solution in DMSO (Cat. No. 500583).
Catalogue Number203729
Brand Family Calbiochem®
Synonyms1,6,7,8,9,11a,12,13,14,14a-Decahydro-1,13-dihydroxy-6-methyl-4H-cyclopent[f]oxacyclotridecin-4-one
References
ReferencesYamagi, R., et al. 2000. Proc. Natl. Acad. Sci. USA 97, 2567.
Moriango, N., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 12856.
Mendez, A.J. 1995. J. Biol. Chem. 270, 5891.
Ridgway, N.D., et al. 1995. J. Biol. Chem. 270, 8023.
Tsai, S.C., et al. 1994. Proc. Natl. Acad. Sci. USA 91, 3063.
Ktistakis, N.Y., et al. 1992. Nature 356, 344.
Product Information
CAS number20350-15-6
ATP CompetitiveN
FormWhite crystalline solid
Hill FormulaC₁₆H₂₄O₄
Chemical formulaC₁₆H₂₄O₄
ReversibleY
Structure formula ImageStructure formula Image
Quality LevelMQ100
Applications
Application(+)-Brefeldin A, Eupenicillium brefeldianum, CAS 20350-15-6, specifically and reversibly blocks translocation of proteins from the endoplasmic reticulum to the Golgi apparatus.
Biological Information
Primary TargetBlocks translocation of proteins from the endoplasmic reticulum (ER) to the Golgi apparatus
Purity≥95% by HPLC
Physicochemical Information
Cell permeableN
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
RTECSGY8410000
Safety Information
R PhraseR: 20/21/22

Harmful by inhalation, in contact with skin and if swallowed.
S PhraseS: 36

Wear suitable protective clothing.
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Harmful
Storage -20°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for 2 months at -20°C.
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

(+)-Brefeldin A, Eupenicillium brefeldianum - CAS 20350-15-6 - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

(+)-Brefeldin A, Eupenicillium brefeldianum - CAS 20350-15-6 - Calbiochem Certificates of Analysis

TitleLot Number
203729

References

Reference overview
Yamagi, R., et al. 2000. Proc. Natl. Acad. Sci. USA 97, 2567.
Moriango, N., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 12856.
Mendez, A.J. 1995. J. Biol. Chem. 270, 5891.
Ridgway, N.D., et al. 1995. J. Biol. Chem. 270, 8023.
Tsai, S.C., et al. 1994. Proc. Natl. Acad. Sci. USA 91, 3063.
Ktistakis, N.Y., et al. 1992. Nature 356, 344.

Citations

Title
  • Michael Tscharntke, et al. (2005) Ras-induced spreading and wound closure in human epidermal keratinocytes. The FASEB Journal in press,.
  • Jenny J.L. Ho, et al. (2003) N-glycosylation is required for the surface localization of MUC17 mucin. 23, 585-592.
  • Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision21-August-2009 RFH
    Synonyms1,6,7,8,9,11a,12,13,14,14a-Decahydro-1,13-dihydroxy-6-methyl-4H-cyclopent[f]oxacyclotridecin-4-one
    DescriptionSpecifically and reversibly blocks translocation of proteins from the endoplasmic reticulum (ER) to the Golgi apparatus without affecting endocytosis or lysosome function. Inhibitor of HDL-mediated cholesterol efflux. Causes disassembly of the Golgi complex and ER swelling in a variety of mammalian cell lines at <40 ng/ml. Inhibits the activity of BIG1 and BIG2, the guanine-nucleotide exchange proteins for ADP-ribosylation factors. Blocks binding of ADP-ribosylation factor to the Golgi and inhibits the GDP-GTP exchange.
    FormWhite crystalline solid
    CAS number20350-15-6
    RTECSGY8410000
    Chemical formulaC₁₆H₂₄O₄
    Structure formulaStructure formula
    Purity≥95% by HPLC
    SolubilityMethanol (2 mg/ml)
    Storage -20°C
    Do Not Freeze Ok to freeze
    Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for 2 months at -20°C.
    Toxicity Harmful
    Merck USA index14, 1369
    ReferencesYamagi, R., et al. 2000. Proc. Natl. Acad. Sci. USA 97, 2567.
    Moriango, N., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 12856.
    Mendez, A.J. 1995. J. Biol. Chem. 270, 5891.
    Ridgway, N.D., et al. 1995. J. Biol. Chem. 270, 8023.
    Tsai, S.C., et al. 1994. Proc. Natl. Acad. Sci. USA 91, 3063.
    Ktistakis, N.Y., et al. 1992. Nature 356, 344.
    Citation
  • Michael Tscharntke, et al. (2005) Ras-induced spreading and wound closure in human epidermal keratinocytes. The FASEB Journal in press,.
  • Jenny J.L. Ho, et al. (2003) N-glycosylation is required for the surface localization of MUC17 mucin. 23, 585-592.