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110116 β1-2,3,4,6-N-Acetylglucosaminidase, Streptococcus pneumoniae, Recombinant, E. coli

110116
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Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
110116-1U Plastic ampoule 1 u
Description
OverviewRecombinant, Streptococcus pneumoniae β1-2,3,4,6-N-Acetylglucosaminidase expressed in E. coli. Catalyzes the hydrolysis of β1-2,3,4,6-linked N-acetylglucosamine from complex carbohydrates. Not known to cleave substituted or branched hexosamines. Does not cleave N-acetylgalactosamine residues.
Catalogue Number110116
Brand Family Calbiochem®
SynonymsN-Acetyl-β-D-glycosaminide-N-acetylglucosaminohydrolase
References
ReferencesPrime, S., et al. 1996. J. Chromatogr. A. 720, 263.
Clarke, V.A., et al. 1995. J. Biol. Chem. 270, 8805.
Dwek, R.A., et al. 1993. Annu. Rev. Biochem. 62, 65.
Product Information
CAS number9012-33-3
Activity≥ 35 units/ml
Unit of DefinitionOne unit is defined as the amount of enzyme that will catalyze the release of 1.0 µmol <i>p</i>-nitrophenol from p-nitrophenyl-β-D-N-acetylglucosaminide per minute at 37°C, pH 5.0.
EC number3.2.1.30
FormLiquid
FormulationIn 25 mM NaCl, 20 mM Tris-HCl, pH 7.5.
Quality LevelMQ100
Applications
Biological Information
Specific Activity≥ 80 units/mg protein
Physicochemical Information
ContaminantsEndo-α-N-acetylgalactosaminidase, α- and β-galactosidase, neuraminidases, proteases: none detected.
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Yes
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

β1-2,3,4,6-N-Acetylglucosaminidase, Streptococcus pneumoniae, Recombinant, E. coli SDS

Title

Safety Data Sheet (SDS) 

β1-2,3,4,6-N-Acetylglucosaminidase, Streptococcus pneumoniae, Recombinant, E. coli Certificates of Analysis

TitleLot Number
110116

References

Reference overview
Prime, S., et al. 1996. J. Chromatogr. A. 720, 263.
Clarke, V.A., et al. 1995. J. Biol. Chem. 270, 8805.
Dwek, R.A., et al. 1993. Annu. Rev. Biochem. 62, 65.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision07-April-2008 RFH
SynonymsN-Acetyl-β-D-glycosaminide-N-acetylglucosaminohydrolase
DescriptionRecombinant, Streptococcus pneumoniae β1-2,3,4,6-N-Acetylglucosaminidase expressed in E. coli. Catalyzes the hydrolysis of β1-2,3,4,6-linked N-acetylglucosamine from complex carbohydrates. Not known to cleave substituted or branched hexosamines. Does not cleave N-acetylgalactosamine residues.
FormLiquid
FormulationIn 25 mM NaCl, 20 mM Tris-HCl, pH 7.5.
Recommended reaction conditions50 mM sodium phosphate buffer, pH 5.0.
CAS number9012-33-3
EC number3.2.1.30
ContaminantsEndo-α-N-acetylgalactosaminidase, α- and β-galactosidase, neuraminidases, proteases: none detected.
Specific activity≥ 80 units/mg protein
Activity≥ 35 units/ml
Unit definitionOne unit is defined as the amount of enzyme that will catalyze the release of 1.0 µmol p-nitrophenol from p-nitrophenyl-β-D-N-acetylglucosaminide per minute at 37°C, pH 5.0.
Storage +2°C to +8°C
Do Not Freeze Yes
Toxicity Standard Handling
ReferencesPrime, S., et al. 1996. J. Chromatogr. A. 720, 263.
Clarke, V.A., et al. 1995. J. Biol. Chem. 270, 8805.
Dwek, R.A., et al. 1993. Annu. Rev. Biochem. 62, 65.