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345804 β1-3,6-Galactosidase, Recombinant, E. coli

345804
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Products

Catalogue NumberPackaging Qty/Pack
345804-2U Plastic ampoule 2 u
Description
OverviewRecombinant, β1-3,6-Galactosidase expressed in E. coli. Catalyzes the hydrolysis of non-reducing terminal β1,3- and β1,6-linked galactose from complex carbohydrates and glycoproteins. Not known to cleave substituted or branched galactose. Inhibited by fucose attached to penultimate sugar.
Catalogue Number345804
Brand Family Calbiochem®
Synonymsβ1-3,6-D-Galactoside Galactohydrolase
References
ReferencesPrime, S., et al. 1996. J. Chromatogr. A. 720, 263.
Dwek, R.A., et al. 1993. Annu. Rev. Biochem. 62, 65.
Product Information
CAS number9031-11-2
Activity≥30 units/ml
Unit of DefinitionOne unit is defined as the amount of enzyme that will catalyze the release of 1.0 µmol <i>p</i>-nitrophenol from <i>p</i>-nitrophenyl-β-D-galactopyranoside per min at 37°C, pH 5.0.
EC number3.2.1.23
FormLiquid
FormulationIn 25 mM NaCl, 20 mM Tris-HCl, pH 7.5.
Quality LevelMQ100
Applications
Biological Information
Specific Activity≥75 units/mg protein
Physicochemical Information
ContaminantsProteases: none detected
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Yes
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

β1-3,6-Galactosidase, Recombinant, E. coli SDS

Title

Safety Data Sheet (SDS) 

β1-3,6-Galactosidase, Recombinant, E. coli Certificates of Analysis

TitleLot Number
345804

References

Reference overview
Prime, S., et al. 1996. J. Chromatogr. A. 720, 263.
Dwek, R.A., et al. 1993. Annu. Rev. Biochem. 62, 65.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision30-June-2008 RFH
Synonymsβ1-3,6-D-Galactoside Galactohydrolase
DescriptionRecombinant, β1-3,6-Galactosidase expressed in E. coli. Catalyzes the hydrolysis of non-reducing terminal β1,3-and β1,6-linked galactose from complex carbohydrates and glycoproteins. Not known to cleave substituted or branched galactose. Inhibited by fucose attached to penultimate sugar.
FormLiquid
FormulationIn 25 mM NaCl, 20 mM Tris-HCl, pH 7.5.
Recommended reaction conditions50 mM sodium phosphate buffer, pH 5.0.
CAS number9031-11-2
EC number3.2.1.23
ContaminantsProteases: none detected
Specific activity≥75 units/mg protein
Activity≥30 units/ml
Unit definitionOne unit is defined as the amount of enzyme that will catalyze the release of 1.0 µmol p-nitrophenol from p-nitrophenyl-β-D-galactopyranoside per min at 37°C, pH 5.0.
Storage +2°C to +8°C
Do Not Freeze Yes
Toxicity Standard Handling
ReferencesPrime, S., et al. 1996. J. Chromatogr. A. 720, 263.
Dwek, R.A., et al. 1993. Annu. Rev. Biochem. 62, 65.