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100081 14-3-3 Antagonist I, 2-5 - Calbiochem

100081
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Overview

Replacement Information

Key Spec Table

Empirical Formula
C₂₀H₂₃N₆O₈P

Products

Catalogue NumberPackaging Qty/Pack
100081-5MG Glass bottle 5 mg
Description
OverviewA cell-permeable dipeptidyl-phosphoserine compound that effectively disrupts 14-3-3 interaction with its ligands (IC50 = 2.6 µM against TMR-GG-RLSHpSSLPG binding to human 14-3-3ς), including Raf-1 and p53. Shown to induce caspase-3 activation and apoptotic cell death in human lung adenocarcinoma A549 cultures (100µM) in a time-dependent manner.
Catalogue Number100081
Brand Family Calbiochem®
Synonyms(S)-2-(2-(4-Azidobenzamido)acetamido)-3-((4-hydroxyphenethyl)amino)-3-oxopropyl-dihydrogen phosphate
References
ReferencesWu, H., et al. 2010. Angew. Chem. Int. Ed. 49, 6528.
Product Information
FormPale yellow to beige solid
Hill FormulaC₂₀H₂₃N₆O₈P
Chemical formulaC₂₀H₂₃N₆O₈P
Hygroscopic Hygroscopic
Structure formula ImageStructure formula Image
Quality LevelMQ100
Applications
Biological Information
Purity≥90% by HPLC
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Shipped with Blue Ice or with Dry Ice
Toxicity Standard Handling
Storage -20°C
Protect from Light Protect from light
Hygroscopic Hygroscopic
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications

Documentation

14-3-3 Antagonist I, 2-5 - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

14-3-3 Antagonist I, 2-5 - Calbiochem Certificates of Analysis

TitleLot Number
100081

References

Reference overview
Wu, H., et al. 2010. Angew. Chem. Int. Ed. 49, 6528.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision22-March-2012 JSW
Synonyms(S)-2-(2-(4-Azidobenzamido)acetamido)-3-((4-hydroxyphenethyl)amino)-3-oxopropyl-dihydrogen phosphate
DescriptionA cell-permeable dipeptidyl-phosphoserine compound that targets 14-3-3 phosphopeptide binding site and effectively competes against 14-3-3 ligands, including Raf-1 and p53, binding to 14-3-3 (IC50 = 2.6 µM against TMR-GG-RLSHpSSLPG binding to human 14-3-3ς). Shown to induce caspase-3 activation and apoptotic cell death in human lung adenocarcinoma A549 cultures (live cell population after 12 h 100 µM drug treatment = 30% of control). Stability study using A549 cell lysates (100 µM drug in 1 µg/ml lysate) reveals a dephosphorylation t1/2 of ~ 6 hrs and no detectable proteolytic degradation up to 12 hrs.
FormPale yellow to beige solid
Intert gas (Yes/No) Packaged under inert gas
Chemical formulaC₂₀H₂₃N₆O₈P
Structure formulaStructure formula
Purity≥90% by HPLC
SolubilityDMSO (50 mg/ml)
Storage -20°C
Hygroscopic
Protect from light
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Toxicity Standard Handling
ReferencesWu, H., et al. 2010. Angew. Chem. Int. Ed. 49, 6528.