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345806 β1,4-Galactosidase, Streptococcus pneumoniae, Recombinant, E. coli

345806
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Products

Catalogue NumberPackaging Qty/Pack
345806-50MIU Plastic ampoule 50 miu
Description
OverviewRecombinant, Streptococcus pneumoniae β1,4-Galactosidase expressed in E. coli. Catalyzes the hydrolysis of non-reducing terminal β1,4-linked galactose. This enzyme is not known to cleave substituted or branched galactose. Inhibited by fucose attached to penultimate sugar.
Note: 1 mU = 1 milliunit.
Catalogue Number345806
Brand Family Calbiochem®
Synonymsβ1,4-D-Galactoside Galactohydrolase
References
ReferencesKierman, U.A., et al. 2004. Proteomincs 4, 1825.
Prime, S., et al. 1996. J. Chromatogr. A. 720, 263.
Dwek, R.A., et al. 1993. Annu. Rev. Biochem. 62, 65.
Product Information
CAS number9031-11-2
Activity≥1.5 units/ml
Unit of DefinitionOne unit is defined as the amount of enzyme that will catalyze the release of 1.0 µmol <i>p</i>-nitrophenol from <i>p</i>-nitrophenyl-β-D-galactopyranoside per min at 37°C, pH 5.0.
EC number3.2.1.23
FormLiquid
FormulationIn 25 mM NaCl, 20 mM Tris-HCl, pH 7.5. Sterile solution.
Quality LevelMQ200
Applications
Biological Information
Specific Activity≥6 units/mg protein
Physicochemical Information
ContaminantsN-acetylglucosaminidase, α-galactosidase, α-mannosidase, neuraminidases, proteases: none detected. Recommended reaction buffer: 50 mM sodium phosphate buffer, pH 6.0
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Yes
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

β1,4-Galactosidase, Streptococcus pneumoniae, Recombinant, E. coli SDS

Title

Safety Data Sheet (SDS) 

β1,4-Galactosidase, Streptococcus pneumoniae, Recombinant, E. coli Certificates of Analysis

TitleLot Number
345806

References

Reference overview
Kierman, U.A., et al. 2004. Proteomincs 4, 1825.
Prime, S., et al. 1996. J. Chromatogr. A. 720, 263.
Dwek, R.A., et al. 1993. Annu. Rev. Biochem. 62, 65.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision30-June-2008 RFH
Synonymsβ1,4-D-Galactoside Galactohydrolase
DescriptionRecombinant, Streptococcus pneumoniae β1,4-Galactosidase expressed in E. coli. Catalyzes the hydrolysis of non-reducing terminal β1,4-linked galactose. This enzyme is not known to cleave substituted or branched galactose. Inhibited by fucose attached to penultimate sugar.
FormLiquid
FormulationIn 25 mM NaCl, 20 mM Tris-HCl, pH 7.5. Sterile solution.
Recommended reaction conditions50 mM sodium phosphate buffer, pH 6.0.
CAS number9031-11-2
EC number3.2.1.23
ContaminantsN-acetylglucosaminidase, α-galactosidase, α-mannosidase, neuraminidases, proteases: none detected. Recommended reaction buffer: 50 mM sodium phosphate buffer, pH 6.0
Specific activity≥6 units/mg protein
Activity≥1.5 units/ml
Unit definitionOne unit is defined as the amount of enzyme that will catalyze the release of 1.0 µmol p-nitrophenol from p-nitrophenyl-β-D-galactopyranoside per min at 37°C, pH 5.0.
Storage +2°C to +8°C
Do Not Freeze Yes
Toxicity Standard Handling
ReferencesKierman, U.A., et al. 2004. Proteomincs 4, 1825.
Prime, S., et al. 1996. J. Chromatogr. A. 720, 263.
Dwek, R.A., et al. 1993. Annu. Rev. Biochem. 62, 65.