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178375 APO-10 - CAS 2190-95-6 - Calbiochem

178375
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Overview

Replacement Information

Key Spec Table

Empirical FormulaCAS #
C₁₂H₂₇PO2190-95-6

Products

Catalogue NumberPackaging Qty/Pack
178375-1GM Plastic ampoule 1 gm
Description
OverviewNovel non-ionic detergent useful for plasmid DNA isolation. Plasmid DNA isolated using APO-10 does not require the use of phenol/chloroform or proprietary resins. Provides excellent yields of high quality DNA. Allows plasmid DNA to be sequenced through difficult regions without the need to subclone into single-stranded vectors.
Catalogue Number178375
Brand Family Calbiochem®
SynonymsDimethyldecylphosphine Oxide
References
ReferencesLi, L., et al. 2003. Hypertension 42, 997.
Altschuler, M., et al. 1994. Biotechniques 17, 434.
Product Information
CAS number2190-95-6
Aggregation Number131
CMC4.6 mM
FormWhite solid
Hill FormulaC₁₂H₂₇PO
Chemical formulaC₁₂H₂₇PO
Structure formula ImageStructure formula Image
Quality LevelMQ100
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
R PhraseR: 36/37/38

Irritating to eyes, respiratory system and skin.
S PhraseS: 26-36

In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing.
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Irritant
Storage +15°C to +30°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, store at room temperature (20°C). Stock solutions are stable for up to 6 months at room temperature.
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

APO-10 - CAS 2190-95-6 - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

APO-10 - CAS 2190-95-6 - Calbiochem Certificates of Analysis

TitleLot Number
178375

References

Reference overview
Li, L., et al. 2003. Hypertension 42, 997.
Altschuler, M., et al. 1994. Biotechniques 17, 434.

Brochure

Title
Biological Detergents Brochure
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision15-April-2008 RFH
SynonymsDimethyldecylphosphine Oxide
DescriptionNovel non-ionic detergent useful for plasmid DNA isolation (aggregation number: 131; CMC: 4.6 mM). Plasmid DNA isolated using APO-10 does not require the use of phenol/chloroform or proprietary resins. Provides excellent yields of high quality DNA. Allows plasmid DNA to be sequenced through difficult regions without the need to subclone into single-stranded vectors.
FormWhite solid
Recommended reaction conditions
Protocol for Plasmid DNA Isolation using APO-10 1. Centrifuge 1.5 ml of bacterial culture at 12,000 x g for 2 min. 2. Resuspend the pellet in 100 µl of 50 mM glucose, 25 mM Tris-HCl, 10 mM EDTA, 10 µg/ml RNase, pH 8.0. 3. Add 200 µl of 200 mM NaOH and 1% APO-10. Mix gently by inverting. Incubate at room temperature for 5 min. 4. Add 150 µl of 3 M potassium acetate buffer, pH 4.8. Mix gently by inverting. Incubate at room temperature for 5 min. 5. Centrifuge sample for 15 min at 4°C. Collect the supernatant. 6. Add 1 ml of 100% ethanol to the supernatant, mix, and centrifuge for 15 min at 4°C. Wash the pellet in 70% ethanol and dry. 7. The DNA pellet can be resuspended in 25 µl TE buffer (10 mM Tris-HCl, pH 8.0, 1 mM EDTA). RNase A can be added to digest any RNA.
CAS number2190-95-6
Chemical formulaC₁₂H₂₇PO
Structure formulaStructure formula
SolubilityH₂O (10 mg/ml)
Storage +15°C to +30°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, store at room temperature (20°C). Stock solutions are stable for up to 6 months at room temperature.
Toxicity Irritant
ReferencesLi, L., et al. 2003. Hypertension 42, 997.
Altschuler, M., et al. 1994. Biotechniques 17, 434.