Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

194933 AZOCOLL™ Substrate, >100 Mesh - CAS 53092-90-3 - Calbiochem

194933
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Products

Catalogue NumberPackaging Qty/Pack
194933-1GM Plastic ampoule 1 gm
194933-5GM Glass bottle 5 gm
Description
OverviewGeneral proteolytic substrate. Smaller particle size provides a rapid, simple method for quantitative determination of protease content in aqueous solutions. Color yield (520 nm): >1.0; blank (520 nm, 50 mg/5 ml buffer, 10 minutes at 37°C, pH 7.0): <0.3. Mesh: >100.
Catalogue Number194933
Brand Family Calbiochem®
References
ReferencesPoilane, I., et al. 1998. Can. J. Microbiol. 44, 157.
Braganza, V.J., and Simmons, W.H. 1991. Biochemistry 30, 4997.
Product Information
CAS number53092-90-3
ATP CompetitiveN
FormDark red solid
ReversibleN
Quality LevelMQ100
Applications
Biological Information
Primary TargetGeneral proteolytic substrate
Physicochemical Information
Cell permeableN
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +15°C to +30°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

AZOCOLL™ Substrate, >100 Mesh - CAS 53092-90-3 - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

AZOCOLL™ Substrate, >100 Mesh - CAS 53092-90-3 - Calbiochem Certificates of Analysis

TitleLot Number
194933

References

Reference overview
Poilane, I., et al. 1998. Can. J. Microbiol. 44, 157.
Braganza, V.J., and Simmons, W.H. 1991. Biochemistry 30, 4997.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision31-October-2016 JSW
DescriptionGeneral proteolytic substrate. Smaller particle size provides a rapid, simple method for quantitative determination of protease content in aqueous solutions. Color yield (520 nm): >1.0; blank (520 nm, 5 mg/ml buffer, 10 min at 37°C, pH 7.0): <0.3. Mesh: >100.
FormDark red solid
Recommended reaction conditions
Assay Protocol for Protease Activity 1. Dissolve 50 mg AZOCOLL® in 5 ml of buffer solution (e.g., PBS, pH 7.0) containing the proteolytic enzyme. 2. Incubate in stoppered tubes for 15 min at 37°C, agitating gently at 5 min intervals. 3. Cool the tubes in ice water and filter the contents through Whatman No. 1 filter paper. 4. Measure the absorbance at 520 nm, subtracting the blank value. 5. Plot absorbance versus protease activity. A linear relationship exists between protease concentration and the rate of dye liberation. Note: The rate of color yield from AZOCOLL® will vary with the individual proteases tested. This protocol is meant to serve as a guideline and may need to be modified for specific applications.
CAS number53092-90-3
SolubilityPBS (10 mg/ml)
Storage +15°C to +30°C
Protect from light
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Toxicity Standard Handling
ReferencesPoilane, I., et al. 1998. Can. J. Microbiol. 44, 157.
Braganza, V.J., and Simmons, W.H. 1991. Biochemistry 30, 4997.