Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

124020 Akt Inhibitor X - CAS 925681-41-0 - Calbiochem

124020
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Empirical FormulaCAS #
C₂₀H₂₅ClN₂O • HCl925681-41-0

Products

Catalogue NumberPackaging Qty/Pack
124020-5MG Plastic ampoule 5 mg
Description
OverviewA cell-permeable, reversible, and selective inhibitor of the phosphorylation of Akt and its in vitro kinase activity (complete inhibition <5 µM) with minimal effect on PI 3-K, PDK1, or SGK1. Shown to suppress growth of Rh (rhabdomyosarcoma) cell lines (IC50 = 2-5 µM), inhibit IGF-I-stimulated nuclear translocation of Akt, and prevent phosphorylation of the downstream targets, mTOR, p70S6 kinase, and S6 ribosomal protein. Unlike Akti1/2 (Cat. No. 124018), the mode of inhibition is not PH domain-dependent. Also shown to induce neuronal autophagy in an Akt- and mTOR-independent manner and enhances the clearance of misfolded protein. Also available as a 20 mM solution in H2O(Cat. No. 124039).
Catalogue Number124020
Brand Family Calbiochem®
Synonyms10-DEBC HC, 10-(4ʹ-(N-diethylamino)butyl)-2-chlorophenoxazine, HCl, 10-NCP
References
ReferencesTsvetkov, A.S., et al. 2010. Proc. Natl. Acad. Sci. USA in press.
Thimmaiah, K.N., et al. 2005. J. Biol. Chem. 280, 31924.
Product Information
CAS number925681-41-0
ATP CompetitiveN
FormWhite solid
Hill FormulaC₂₀H₂₅ClN₂O • HCl
Chemical formulaC₂₀H₂₅ClN₂O • HCl
Hygroscopic Hygroscopic
ReversibleY
Structure formula ImageStructure formula Image
Quality LevelMQ100
Applications
ApplicationAkt Inhibitor X, CAS 925681-41-0, is a cell-permeable, reversible & selective inhibitor of Akt phosphorylation and its in vitro kinase activity. Has minimal effect on PI 3-K, PDK1, or SGK1.
Biological Information
Primary TargetAkt
Primary Target IC<sub>50</sub><5 µM against Akt ; 2-5 µM against growth of Rh (rhabdomyosarcoma) cell lines
Secondary targetRn cell lines (IC₅₀ = 2-5 µM)
Purity≥95% by HPLC
Physicochemical Information
Cell permeableY
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Irritant
Storage +2°C to +8°C
Protect from Light Protect from light
Hygroscopic Hygroscopic
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications

Documentation

Akt Inhibitor X - CAS 925681-41-0 - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

Akt Inhibitor X - CAS 925681-41-0 - Calbiochem Certificates of Analysis

TitleLot Number
124020

References

Reference overview
Tsvetkov, A.S., et al. 2010. Proc. Natl. Acad. Sci. USA in press.
Thimmaiah, K.N., et al. 2005. J. Biol. Chem. 280, 31924.

Brochure

Title
Akt
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision14-September-2010 JSW
Synonyms10-DEBC HC, 10-(4ʹ-(N-diethylamino)butyl)-2-chlorophenoxazine, HCl, 10-NCP
DescriptionA cell-permeable, reversible, and selective inhibitor of the phosphorylation of Akt and its in vitro kinase activity (complete inhibition < 5 µM) with minimal effect on PI 3-K, PDK1, or SGK1. Shown to suppress growth of Rh (rhabdomyosarcoma) cell lines (IC50 = 2-5 μM), inhibit IGF-I-stimulated nuclear translocation of Akt, and prevent phosphorylation of the downstream targets, mTOR, p70S6 kinase, and S6 ribosomal protein. Unlike Akti1/2 (Cat. No. 124018), the mode of inhibition is not PH domain-dependent. Also shown to induce neuronal autophagy in an Akt- and mTOR-independent manner and enhances the clearance of misfolded protein.
FormWhite solid
Intert gas (Yes/No) Packaged under inert gas
CAS number925681-41-0
Chemical formulaC₂₀H₂₅ClN₂O • HCl
Structure formulaStructure formula
Purity≥95% by HPLC
SolubilityH₂O (1 mg/ml)
Storage +2°C to +8°C
Hygroscopic
Protect from light
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Toxicity Irritant
ReferencesTsvetkov, A.S., et al. 2010. Proc. Natl. Acad. Sci. USA in press.
Thimmaiah, K.N., et al. 2005. J. Biol. Chem. 280, 31924.