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113200 Anti-α-Actin Mouse mAb (1A4)

113200
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
Bab, H, RbMMonoclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
113200-500UL Plastic ampoule 500 ul
Description
OverviewRecognizes α-actin in smooth muscle cells of blood vessels and parenchymal tissue of the intestine, testis, and ovary. Does not recognize actin from fibroblasts (β- and γ-cytoplasmic), striated muscle (α-sarcomeric), or myocardium (α-myocardial).
  • Antibody Target Gene Symbol: ACTA2
  • Target Synonym: 0610041G09RIK, a-SMA, AAT6, ACTSA, Actvs, ALPHA ACTIN VSM, ALPHA SMA, ALPHA SMOOTH MUSCLE ACTIN, ALPHA-ACTIN 2, FLJ45296, MGC127800, RGD: 621676, Similar to vascular alpha actin, Sm alpha actin, SMalphaA, SMOOTH MUSCLE ALPHA ACTIN
  • Entrez Gene Name: actin, alpha 2, smooth muscle, aorta
  • Hu Entrez ID: 59 (Related Antibodies: 113200)
  • Rat Entrez ID: 81633
  • Catalogue Number113200
    Brand Family Calbiochem®
    References
    ReferencesRannov-Jennsen, L., et al. 1990. Lab Invest. 63, 532.
    Skalli, O., et al. 1989. J. Histochem. Cytochem 37, 315.
    Skalli, O., et al. 1986. J. Cell. Biol. 103, 2787.
    Product Information
    FormLiquid
    FormulationIn 150 mM NaCl, 20 mM Tris-borate, BHC-6 carrier polysaccharides, pH 7.5.
    Negative controlFibroblasts, striated muscle, or myocardium
    Positive controlSmooth muscle cells of blood vessels and parenchymal tissue of intestine, testis, or ovary
    Preservative≤0.1% sodium azide
    Quality LevelMQ100
    Applications
    Key Applications Frozen Sections
    Paraffin Sections
    Application NotesFrozen Sections (1:50-1:75)
    Immunoblotting (see comments)
    Paraffin Sections (1:50-1:75)
    Application CommentsDoes not recognize actin from fibroblasts (β- and γ-cytoplasmic), striated muscle (α-sarcomeric), or myocardium (α-myocardial).Prolonged fixation in buffered formalin destroys the epitope. The antibody is specific for the α-smooth muscle isoform of actin. This antibody is also reported to work for immunoblotting. Variables associated with assay conditions will dictate the proper working dilution.
    Biological Information
    Immunogena synthetic peptide corresponding to amino acids 1-10 of human smooth muscle α-actin.
    ImmunogenHuman Smooth Muscle
    Clone1A4
    HostMouse
    IsotypeIgG2a
    Species Reactivity
    • Baboon
    • Human
    • Rabbit
    Antibody TypeMonoclonal Antibody
    Concentration Label Please refer to vial label for lot-specific concentration
    Physicochemical Information
    Dimensions
    Materials Information
    Toxicological Information
    Safety Information according to GHS
    Safety Information
    Product Usage Statements
    Storage and Shipping Information
    Ship Code Blue Ice Only
    Toxicity Standard Handling
    Storage +2°C to +8°C
    Do not freeze Yes
    Packaging Information
    Transport Information
    Supplemental Information
    Specifications

    Documentation

    Anti-α-Actin Mouse mAb (1A4) SDS

    Title

    Safety Data Sheet (SDS) 

    Anti-α-Actin Mouse mAb (1A4) Certificates of Analysis

    TitleLot Number
    113200

    References

    Reference overview
    Rannov-Jennsen, L., et al. 1990. Lab Invest. 63, 532.
    Skalli, O., et al. 1989. J. Histochem. Cytochem 37, 315.
    Skalli, O., et al. 1986. J. Cell. Biol. 103, 2787.

    Brochure

    Title
    Antibody Sourcebookル Sixth Edition
    Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision17-July-2007 RFH
    ApplicationFrozen Sections (1:50-1:75)
    Immunoblotting (see comments)
    Paraffin Sections (1:50-1:75)
    DescriptionMouse monoclonal antibody. Recognizes α-actin in smooth muscle cells.
    BackgroundActin is a cytoskeletal protein. Smooth muscle actin forms smaller, less ordered contractile units than actin from skeletal or cardiac muscle. The activity and state of assembly of smooth muscle actin is controlled by calcium-regulated phosphorylation of one of the myosin light chains.
    HostMouse
    Immunogen speciesHuman Smooth Muscle
    Immunogena synthetic peptide corresponding to amino acids 1-10 of human smooth muscle α-actin.
    Clone1A4
    IsotypeIgG2a
    Speciesbaboon, human, rabbit
    Positive controlSmooth muscle cells of blood vessels and parenchymal tissue of intestine, testis, or ovary
    Negative controlFibroblasts, striated muscle, or myocardium
    FormLiquid
    FormulationIn 150 mM NaCl, 20 mM Tris-borate, BHC-6 carrier polysaccharides, pH 7.5.
    Concentration Label Please refer to vial label for lot-specific concentration
    Preservative≤0.1% sodium azide
    CommentsDoes not recognize actin from fibroblasts (β- and γ-cytoplasmic), striated muscle (α-sarcomeric), or myocardium (α-myocardial).Prolonged fixation in buffered formalin destroys the epitope. The antibody is specific for the α-smooth muscle isoform of actin. This antibody is also reported to work for immunoblotting. Variables associated with assay conditions will dictate the proper working dilution.
    Storage +2°C to +8°C
    Do Not Freeze Yes
    Toxicity Standard Handling
    ReferencesRannov-Jennsen, L., et al. 1990. Lab Invest. 63, 532.
    Skalli, O., et al. 1989. J. Histochem. Cytochem 37, 315.
    Skalli, O., et al. 1986. J. Cell. Biol. 103, 2787.