Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

171610 Anti-Amyloid Precursor Protein, C-Terminal (751-770) Rabbit pAb

171610
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HRbPolyclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
171610-50UL Plastic ampoule 50 ul
Description
OverviewRecognizes full-length APP and C-terminal soluble products CTFγ (~6 kDa), CTFα (~9 kDa), and CTFβ (~11 kDa).
  • Antibody Target Gene Symbol: APP
  • Target Synonym: AAA, AD1, Adap, AL024401, Amyloid precursor, Amyloidogenic glycoprotein, APP isoform 1, APPI, appican, CTFgamma, CVAP, E030013M08RIK, Nexin II, P3, PN2, PreA4, PROTEASE NEXIN2
  • Entrez Gene Name: amyloid beta (A4) precursor protein
  • Hu Entrez ID: 351 (Related Antibodies: PC729, PC152,PC151, PC150, PC149, NE1012,171609, 171608, 171607, 171606, 171605, 171604, 171603, 171598, 171537)
  • Mu Entrez ID: 11820
  • Rat Entrez ID: 54226
  • Catalogue Number171610
    Brand Family Calbiochem®
    SynonymsAnti-0443, Anti-APP-CT20
    References
    ReferencesPinnix, I., et al. 2001. J. Biol. Chem. 276, 481.
    Product Information
    DeclarationPurchase of this material is for research use only. Users are invited to request a license from Mayo prior to using the 0433 antibody or any derivatives for clinical diagnostic or therapeutic purposes. For license information, please contact: Mayo Medical Ventures, Office of Technology Commercialization (507) 284-8878, 200 First Street SW, Rochester, MN 55905-001.
    FormLiquid
    Quality LevelMQ100
    Applications
    Key Applications Immunoprecipitation
    Immunoblotting (Western Blotting)
    Immunofluorescence
    Immunohistochemistry
    Application NotesImmunoblotting (1:5000-1:20,000)
    Immunofluorescence (1:1000)
    Immunohistochemistry (1:1000)
    Immunoprecipitation (1:1000)
    Application CommentsVariables associated with assay conditions will dictate the proper working dilution.
    Biological Information
    Immunogena synthetic peptide [(C)KMQQNGYENPTYKFFEQMQN] corresponding to amino acids 751-770 of human precursor protein (APP), conjugated to KLH
    ImmunogenHuman
    HostRabbit
    IsotypeIgG
    Species Reactivity
    • Human
    Antibody TypePolyclonal Antibody
    Physicochemical Information
    Dimensions
    Materials Information
    Toxicological Information
    Safety Information according to GHS
    Safety Information
    Product Usage Statements
    Storage and Shipping Information
    Ship Code Ambient Temperature Only
    Toxicity Standard Handling
    Storage -20°C
    Do not freeze Ok to freeze
    Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
    Packaging Information
    Transport Information
    Supplemental Information
    Specifications

    Documentation

    Anti-Amyloid Precursor Protein, C-Terminal (751-770) Rabbit pAb SDS

    Title

    Safety Data Sheet (SDS) 

    Anti-Amyloid Precursor Protein, C-Terminal (751-770) Rabbit pAb Certificates of Analysis

    TitleLot Number
    171610

    References

    Reference overview
    Pinnix, I., et al. 2001. J. Biol. Chem. 276, 481.

    Brochure

    Title
    Alzheimer's Disease Brochure & Technical Guide

    Citations

    Title
  • Jonathan D. J. Wrigley, et al. (2005) Functional overexpression of γ-secretase reveals protease independent trafficking functions and a critical role of lipids for protease activity. Journal of Biological Chemistry 280, 12523-12535.
  • Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision23-July-2007 RFH
    SynonymsAnti-0443, Anti-APP-CT20
    ApplicationImmunoblotting (1:5000-1:20,000)
    Immunofluorescence (1:1000)
    Immunohistochemistry (1:1000)
    Immunoprecipitation (1:1000)
    DescriptionRabbit polyclonal antibody supplied as undiluted serum. Recognizes full-length APP and C-terminal soluble products CTFγ (~6 kDa), CTFα (~9 kDa), and CTFβ (~11 kDa).
    BackgroundAmyloid precursor protein (APP) is a type I integral membrane protein with a large N-terminal extracellular domain, a single transmembrane domain, and a short cytoplasmic tail. APP is cleaved by alpha-secretase to generate sAPPα and CTFα, which is the C-terminal membrane, associated product of α-secretase cleavage. α-secretase cleaves in the Aβ domain. β-secretase cleavage of APP at the N-terminal end of the Aβ sequence results in sAPPβ and CTFβ. Both CTFα and CTFβ are cleaved at the C-terminal end of the Aβ transmembrane domain to generate CTFγ. The antibody (171610) detects CTFγ fragment, which migrates at about 6 kDa on SDS-PAGE gels.
    HostRabbit
    Immunogen speciesHuman
    Immunogena synthetic peptide [(C)KMQQNGYENPTYKFFEQMQN] corresponding to amino acids 751-770 of human precursor protein (APP), conjugated to KLH
    IsotypeIgG
    Specieshuman
    FormLiquid
    CommentsVariables associated with assay conditions will dictate the proper working dilution.
    Storage -20°C
    Do Not Freeze Ok to freeze
    Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
    Toxicity Standard Handling
    ReferencesPinnix, I., et al. 2001. J. Biol. Chem. 276, 481.
    Citation
  • Jonathan D. J. Wrigley, et al. (2005) Functional overexpression of γ-secretase reveals protease independent trafficking functions and a critical role of lipids for protease activity. Journal of Biological Chemistry 280, 12523-12535.