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217570 Anti-CD3 Mouse mAb (UCHT1)

217570
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
217570-100UG Plastic ampoule 100 μg
Description
OverviewRecognizes the ε-chain of the CD3 complex in human peripheral T cells. Can be used to activate T cells expressing CD3ε.
Catalogue Number217570
Brand Family Calbiochem®
References
ReferencesThibault, G. and Bardos, P. 1995. J. Immunol. 154, 3814.
Beverley, P.C., and Callard, R.E. 1981. Eur. J. Immunol. 11, 329.
Product Information
FormLiquid
FormulationIn 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, pH 7.5.
Preservative0.5 mg/ml gentamycin sulfate
Quality LevelMQ100
Applications
Application ReferencesT cell activation Lafont, V., et al. 1999. J. Biol. Chem. 274, 25743.
Key Applications T Cell Co-stimulation
Flow Cytometry
Frozen Sections
Immunoprecipitation
Application NotesFlow Cytometry (5 µg/ml)
Frozen Sections (see comments)
Immunoprecipitation (see comments)
T cell Co-stimulation (see application references)
Application CommentsThis antibody can be used to activate T cells expressing CD3ε (see application reference). FACS was performed on 5 x 105 cultured HPB-MLT human tumor cells. Optimal binding concentration tested was 5 µg/ml when used with secondary reagent goat anti-mouse IgG, FITC conjugated. This antibody is reported to work for immunoprecipitation and for staining frozen sections. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogenhuman thymocytes/Sezary T cells
ImmunogenHuman
CloneUCHT1
HostMouse
Purity>95% immunoglobulin by SDS-PAGE
IsotypeIgG₁
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Ok to freeze
Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Anti-CD3 Mouse mAb (UCHT1) SDS

Title

Safety Data Sheet (SDS) 

Anti-CD3 Mouse mAb (UCHT1) Certificates of Analysis

TitleLot Number
217570

References

Reference overview
Thibault, G. and Bardos, P. 1995. J. Immunol. 154, 3814.
Beverley, P.C., and Callard, R.E. 1981. Eur. J. Immunol. 11, 329.

Citations

Title
  • Renee M. Marshall, et al. (2005) Cyclin T1 expression is regulated by multiple signaling pathways and mechanisms during activation of human peripheral blood lymphocytes. Journal of Immunology 175, 6402-6411.
  • Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision25-July-2007 RFH
    ApplicationFlow Cytometry (5 µg/ml)
    Frozen Sections (see comments)
    Immunoprecipitation (see comments)
    T cell Co-stimulation (see application references)
    DescriptionProtein A purified mouse monoclonal antibody. Recognizes the ~20 kDa ε chain of the CD3 T-cell receptor complex.
    HostMouse
    Immunogen speciesHuman
    Immunogenhuman thymocytes/Sezary T cells
    CloneUCHT1
    IsotypeIgG₁
    Specieshuman
    FormLiquid
    FormulationIn 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, pH 7.5.
    Concentration Label Please refer to vial label for lot-specific concentration
    Purity>95% immunoglobulin by SDS-PAGE
    Preservative0.5 mg/ml gentamycin sulfate
    CommentsThis antibody can be used to activate T cells expressing CD3ε (see application reference). FACS was performed on 5 x 105 cultured HPB-MLT human tumor cells. Optimal binding concentration tested was 5 µg/ml when used with secondary reagent goat anti-mouse IgG, FITC conjugated. This antibody is reported to work for immunoprecipitation and for staining frozen sections. Variables associated with assay conditions will dictate the proper working dilution.
    Storage +2°C to +8°C
    Do Not Freeze Ok to freeze
    Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
    Toxicity Standard Handling
    ReferencesThibault, G. and Bardos, P. 1995. J. Immunol. 154, 3814.
    Beverley, P.C., and Callard, R.E. 1981. Eur. J. Immunol. 11, 329.
    Citation
  • Renee M. Marshall, et al. (2005) Cyclin T1 expression is regulated by multiple signaling pathways and mechanisms during activation of human peripheral blood lymphocytes. Journal of Immunology 175, 6402-6411.
  • Application referencesT cell activation Lafont, V., et al. 1999. J. Biol. Chem. 274, 25743.