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217590 Anti-CD40 Mouse mAb (EA-5)

217590
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
217590-100UG Plastic ampoule 100 μg
Description
OverviewRecognizes the ~44 kDa CD40 protein in Raji cells.
Catalogue Number217590
Brand Family Calbiochem®
References
ReferencesFanslow, W.C., et al. 1992. J. Immunol. 149, 655.
Law, C.L., et al. 1990. Leukemia 4, 732.
Product Information
FormLiquid
FormulationIn 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, 0.5 mg/ml gentamicin sulfate, pH 7.5.
Positive controlRaji cells
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Paraffin Sections
Flow Cytometry
Frozen Sections
Immunoprecipitation
Application NotesFlow cytometry (5 µg/ml)
Frozen Sections (see comments)
Immunoprecipitation (see comments)
Paraffin Sections (see comments)
Application CommentsFACS was performed on 5x105 cultured Raji human tumor cells. Optimal binding concentration tested was 5 µg/ml using FITC-conjugated goat anti-mouse IgG as a secondary antibody. This antibody has also been reported to work for frozen sections, immunoprecipitation, and paraffin sections. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogentransfected murine L cells expressing human CD40
ImmunogenHuman
CloneEA-5
HostMouse
Purity≥95% by SDS-PAGE
IsotypeIgG₁
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Ok to freeze
Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Anti-CD40 Mouse mAb (EA-5) SDS

Title

Safety Data Sheet (SDS) 

Anti-CD40 Mouse mAb (EA-5) Certificates of Analysis

TitleLot Number
217590

References

Reference overview
Fanslow, W.C., et al. 1992. J. Immunol. 149, 655.
Law, C.L., et al. 1990. Leukemia 4, 732.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision15-December-2008 JSW
ApplicationFlow cytometry (5 µg/ml)
Frozen Sections (see comments)
Immunoprecipitation (see comments)
Paraffin Sections (see comments)
DescriptionProtein A purified mouse monoclonal antibody. Recognizes the ~44 kDa CD40 protein.
BackgroundHuman CD40 is a member of the tumor necrosis factor (TNF) family of cell surface receptors, and is found on all B cells (except plasma cells) and some epithelial, carcinoma, and lymphoid dendritic cells. Through binding of its ligand (CD40L/CD154/gp39), CD40 plays an important role in B-cell activation and isotype switching.
HostMouse
Immunogen speciesHuman
Immunogentransfected murine L cells expressing human CD40
CloneEA-5
IsotypeIgG₁
Specieshuman
Positive controlRaji cells
FormLiquid
FormulationIn 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, 0.5 mg/ml gentamicin sulfate, pH 7.5.
Concentration Label Please refer to vial label for lot-specific concentration
Purity≥95% by SDS-PAGE
Preservative≤0.1% sodium azide
CommentsFACS was performed on 5x105 cultured Raji human tumor cells. Optimal binding concentration tested was 5 µg/ml using FITC-conjugated goat anti-mouse IgG as a secondary antibody. This antibody has also been reported to work for frozen sections, immunoprecipitation, and paraffin sections. Variables associated with assay conditions will dictate the proper working dilution.
Storage +2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
Toxicity Standard Handling
ReferencesFanslow, W.C., et al. 1992. J. Immunol. 149, 655.
Law, C.L., et al. 1990. Leukemia 4, 732.