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217594 Anti-CD44 Rat mAb (A020)

217594
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
B, Ca, H, M, Porcine, RbRMonoclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
217594-100UL Plastic ampoule 100 ul
Description
OverviewRecognizes the ~85-95 kDa CD44 protein (gp90) in human and GP85 or Pgp-1 in mouse.
Catalogue Number217594
Brand Family Calbiochem®
References
ReferencesIida, N., and Bourguignon, L.Y.W. 1995. J. Cell Physiol. 162, 121.
Bourguignon, L.Y.W., et al. 1993. J. Immunol. 151, 6634.
Product Information
FormLiquid
FormulationIn 150 mM NaCl, 10 mM sodium phosphate, pH 7.5.
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Application ReferencesNeutralization Studies Bourguignon, L.Y.W., et al. 2007. J. Biol. Chem. 282, 1265. Bourguignon, L.Y.W., et al. 2006. J. Biol. Chem. 281 14026.
Key Applications Immunofluorescence
Immunohistochemistry
Immunoprecipitation
Radioimmunoassay
Enzyme-Linked Immunosorbent Assay
Flow Cytometry
Immunoblotting (Western Blotting)
Application NotesELISA (1:2000)
Flow Cytometry (1:1000)
Immunoblotting (1:1000)
Immunofluorescence (1:200)
Immunohistochemistry (1:250)
Immunoprecipitation (1:200)
Neutralization Studies (see comments and application references)
Radioimmunoassay (1:5000)
Application CommentsThe antibody specifically recognizes and reacts with a common determinant of the CD44 class of glycoproteins. This antibody has been reported to block the interaction between hyaluronan and CD44 (see application references). Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogenpurified human lymphocyte CD44
ImmunogenHuman
CloneA020
HostRat
IsotypeIgG2b
Species Reactivity
  • Bovine
  • Canine
  • Human
  • Mouse
  • Porcine
  • Rabbit
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Anti-CD44 Rat mAb (A020) SDS

Title

Safety Data Sheet (SDS) 

Anti-CD44 Rat mAb (A020) Certificates of Analysis

TitleLot Number
217594

References

Reference overview
Iida, N., and Bourguignon, L.Y.W. 1995. J. Cell Physiol. 162, 121.
Bourguignon, L.Y.W., et al. 1993. J. Immunol. 151, 6634.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision25-July-2007 RFH
ApplicationELISA (1:2000)
Flow Cytometry (1:1000)
Immunoblotting (1:1000)
Immunofluorescence (1:200)
Immunohistochemistry (1:250)
Immunoprecipitation (1:200)
Neutralization Studies (see comments and application references)
Radioimmunoassay (1:5000)
DescriptionRat monoclonal antibody purified by ammonium sulfate precipitation and DEAE chromatography. Recognizes the ~85-95 kDa CD44 protein.
HostRat
Immunogen speciesHuman
Immunogenpurified human lymphocyte CD44
CloneA020
IsotypeIgG2b
Speciesbovine, canine, human, mouse, porcine, rabbit
FormLiquid
FormulationIn 150 mM NaCl, 10 mM sodium phosphate, pH 7.5.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsThe antibody specifically recognizes and reacts with a common determinant of the CD44 class of glycoproteins. This antibody has been reported to block the interaction between hyaluronan and CD44 (see application references). Variables associated with assay conditions will dictate the proper working dilution.
Storage -20°C
Avoid freeze/thaw
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesIida, N., and Bourguignon, L.Y.W. 1995. J. Cell Physiol. 162, 121.
Bourguignon, L.Y.W., et al. 1993. J. Immunol. 151, 6634.
Application referencesNeutralization Studies Bourguignon, L.Y.W., et al. 2007. J. Biol. Chem. 282, 1265. Bourguignon, L.Y.W., et al. 2006. J. Biol. Chem. 281 14026.