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217657 Anti-CD49f Mouse mAb (BQ16)

217657
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
217657-100UG Plastic ampoule 100 μg
Description
OverviewRecognizes the ~150 kDa CD49f (α6 integrin) in Raji cells.
Catalogue Number217657
Brand Family Calbiochem®
SynonymsAnti-VLA-6
References
ReferencesBerditchevski, F., et al. 1995. J. Biol. Chem. 270, 17784.
Liebert, M., et al. 1993. Hybridoma 12, 67.
Product Information
FormLiquid
FormulationIn 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, pH 7.5.
Positive controlRaji cells
Preservative0.5 mg/ml gentamycin sulfate
Quality LevelMQ100
Applications
Key Applications Immunoprecipitation
Flow Cytometry
Frozen Sections
Immunoblotting (Western Blotting)
Application NotesFlow Cytometry (10 µg/ml)
Frozen Sections (see comments)
Immunoblotting (see comments)
Immunoprecipitation (see comments)
Application CommentsThis antibody has also been reported to work for immunoblotting, immunoprecipitation, and frozen sections. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
ImmunogenUM-UC-9 cells (human bladder cancer cell line)
ImmunogenHuman
CloneBQ16
HostMouse
Purity>95% by SDS-PAGE
IsotypeIgG₁
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Anti-CD49f Mouse mAb (BQ16) SDS

Title

Safety Data Sheet (SDS) 

Anti-CD49f Mouse mAb (BQ16) Certificates of Analysis

TitleLot Number
217657

References

Reference overview
Berditchevski, F., et al. 1995. J. Biol. Chem. 270, 17784.
Liebert, M., et al. 1993. Hybridoma 12, 67.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision25-July-2007 RFH
SynonymsAnti-VLA-6
ApplicationFlow Cytometry (10 µg/ml)
Frozen Sections (see comments)
Immunoblotting (see comments)
Immunoprecipitation (see comments)
DescriptionProtein A purified mouse monoclonal antibody. Recognizes the ~150 kDa CD49f (VLA-6) protein.
BackgroundHuman CD49f molecules are integrin α6 chains that are expressed as heterodimers with CD29 (β1-integrin) or CD104 (β4-integrin) and function as adhesion receptors. CD49f is expressed on platelets, monocytes, T lymphocytes, and thymocytes. The ligands for CD49/CD29 include laminin and invasin. CD49f has been shown to associate with CD63.
HostMouse
Immunogen speciesHuman
ImmunogenUM-UC-9 cells (human bladder cancer cell line)
CloneBQ16
IsotypeIgG₁
Specieshuman
Positive controlRaji cells
FormLiquid
FormulationIn 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, pH 7.5.
Concentration Label Please refer to vial label for lot-specific concentration
Purity>95% by SDS-PAGE
Preservative0.5 mg/ml gentamycin sulfate
CommentsThis antibody has also been reported to work for immunoblotting, immunoprecipitation, and frozen sections. Variables associated with assay conditions will dictate the proper working dilution.
Storage +2°C to +8°C
Do Not Freeze Ok to freeze
Toxicity Standard Handling
ReferencesBerditchevski, F., et al. 1995. J. Biol. Chem. 270, 17784.
Liebert, M., et al. 1993. Hybridoma 12, 67.